| 產品編號 | bsm-30092M-FITC |
| 英文名稱1 | Mouse Anti-human CD3/FITC Conjugated antibody |
| 中文名稱 | FITC標記的小鼠抗人CD3單克隆抗體 |
| 別 名 | CD3 epsilon; CD3e; CD3e antigen epsilon polypeptide (TiT3 complex); CD3E antigen epsilon polypeptide; CD3E antigen, epsilon subunit; CD3e molecule epsilon; CD3e molecule, epsilon (CD3 TCR complex); CD3e molecule, epsilon (CD3-TCR complex); CD3E_HUMAN; IMD18; T cell antigen receptor complex epsilon subunit of T3; T cell surface antigen T3/Leu 4 epsilon chain; T cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE. |
| 規格價格 | 25T/220元 購買 50T/360元 購買 100T/580元 購買 大包裝/詢價 |
| 說 明 書 | 25T 50T 100T |
| 研究領域 | 腫瘤 細胞生物 免疫學 細胞表面分子 t-淋巴細胞 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | HIT3b |
| 交叉反應 | Human, |
| 產品應用 | Flow-Cyt=10ul/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD3 |
| 亞 型 | Mouse IgG2a, k |
| 純化方法 | affinity purified by Protein G |
| 儲 存 液 | 0.01M PBS, 0.5%BSA, 0.03% Proclin300 |
| 保存條件 | Store at 2-8℃. Protect from light. Avoid freezing. |
| 產品介紹 |
background: CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2. Function: The CD3 complex mediates signal transduction. Subunit: he TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. Subcellular Location: Membrane; Single-pass type I membrane protein. Similarity: Contains 1 Ig-like (immunoglobulin-like) domain. Contains 1 ITAM domain. Database links: Entrez Gene: 916 Human Entrez Gene: 12501 Mouse Omim: 186830 Human SwissProt: P07766 Human SwissProt: P22646 Mouse Unigene: 3003 Human Unigene: 210361 Mouse
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. CD3 epsilon 參與T淋巴細胞介導,主要用于免疫排斥方面的研究. |
| 產品圖片 |
scatter diagram showing peripheral blood lymphocytes stained with bsm-30092M-FITC. The cells were incubated with the antibody (bsm-30092M-FITC, 5 μl/Test) for 30 min at 22°C. Acquisition of >10,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
