產(chǎn)品編號(hào) | bsm-33133M-Gold |
英文名稱(chēng)1 | Mouse Anti-TAP-Tag/Gold Conjugated antibody |
中文名稱(chēng) | 膠體金標(biāo)記的TAP-Tag(標(biāo)簽)單克隆抗體 |
別 名 | TAP Tag; TAPTag; Tandem affinity purification |
規(guī)格價(jià)格 | 100ul/2980元 購(gòu)買(mǎi) 大包裝/詢(xún)價(jià) |
說(shuō) 明 書(shū) | 100ul(10nm 15nm 35nm) |
產(chǎn)品類(lèi)型 | 標(biāo)簽抗體 |
研究領(lǐng)域 | |
抗體來(lái)源 | Mouse |
克隆類(lèi)型 | Monoclonal |
克 隆 號(hào) | Mix-mA? |
交叉反應(yīng) | Recombinant protein |
產(chǎn)品應(yīng)用 | IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
性 狀 | Lyophilized or Liquid |
濃 度 | 0.4mg/ml |
免 疫 原 | Recombinant TAP-Tag |
亞 型 | IgG |
純化方法 | affinity purified by Protein G |
儲(chǔ) 存 液 | 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. |
保存條件 | Store at 2-8 oC for 3-6 months. Avoid repeated freeze/thaw cycles. |
產(chǎn)品介紹 |
background: Tandem affinity purification (TAP) is a purification technique for studying protein–protein interactions. It involves creating a fusion protein with a designed piece, the TAP tag, on the end. The original TAP method involves the fusion of the TAP tag to the C-terminus of the protein under study. The TAP tag consists of calmodulin binding peptide (CBP) from the N-terminal, followed by tobacco etch virus protease (TEV protease) cleavage site and Protein A, which binds tightly to IgG. The relative order of the modules of the tag is important because Protein A needs to be at the extreme end of the fusion protein so that the entire complex can be retrieved using an IgG matrix. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |