| 產品編號 | bs-7499R-BF647 |
| 英文名稱1 | Rabbit Anti-Histone H4 (mono methyl K20)/BF647 Conjugated antibody |
| 中文名稱 | BF647標記的組蛋白H4（單甲基K20）抗體 |
| 別 名 | H4F2; H4FN; HIST1H4; HIST2H4; HISTH4H4; methyl histone H4; histone H4; H4_HUMAN; Osteogenic growth peptide; OGP. |
| 規格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 產品類型 | 甲基化抗體 |
| 研究領域 | 腫瘤 細胞生物 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human, Cow, Horse, ) |
| 產品應用 | ICC=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 11kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised methylpeptide derived from human Histone H4 around the methylation site of mono methyl K20 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Jul 2008] Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subcellular Location: Nucleus. Chromosome. Post-translational modifications: Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin. Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation. Similarity: Belongs to the histone H4 family. Database links: Entrez Gene: 121504 Human Entrez Gene: 554313 Human Entrez Gene: 8294 Human Entrez Gene: 8359 Human Entrez Gene: 8360 Human Entrez Gene: 8361 Human Entrez Gene: 8362 Human Entrez Gene: 8363 Human Entrez Gene: 8364 Human Entrez Gene: 8365 Human Entrez Gene: 8366 Human Entrez Gene: 8367 Human Entrez Gene: 8368 Human Entrez Gene: 8370 Human Entrez Gene: 100041230 Mouse Entrez Gene: 100862646 Mouse Entrez Gene: 319155 Mouse Entrez Gene: 319156 Mouse Entrez Gene: 319157 Mouse Entrez Gene: 319158 Mouse Entrez Gene: 319159 Mouse Entrez Gene: 319160 Mouse Entrez Gene: 319161 Mouse Entrez Gene: 320332 Mouse Entrez Gene: 326619 Mouse Entrez Gene: 326620 Mouse Entrez Gene: 69386 Mouse Entrez Gene: 97122 Mouse GenBank: NM_003548 Human Omim: 142750 Human SwissProt: P84040 Fruit fly (Drosophila melanogaster) SwissProt: P02304 Human SwissProt: P62805 Human SwissProt: P02304 Mouse SwissProt: P62806 Mouse SwissProt: P09322 Schizosaccharomyces pombe Unigene: 21500 Fruit fly (Drosophila melanogaster) Unigene: 29514 Fruit fly (Drosophila melanogaster) Unigene: 29527 Fruit fly (Drosophila melanogaster) Unigene: 30219 Fruit fly (Drosophila melanogaster) Unigene: 30220 Fruit fly (Drosophila melanogaster) Unigene: 30221 Fruit fly (Drosophila melanogaster) Unigene: 30223 Fruit fly (Drosophila melanogaster) Unigene: 30868 Fruit fly (Drosophila melanogaster) Unigene: 30869 Fruit fly (Drosophila melanogaster) Unigene: 30871 Fruit fly (Drosophila melanogaster) Unigene: 30872 Fruit fly (Drosophila melanogaster) Unigene: 30873 Fruit fly (Drosophila melanogaster) Unigene: 30876 Fruit fly (Drosophila melanogaster) Unigene: 33873 Fruit fly (Drosophila melanogaster) Unigene: 5747 Fruit fly (Drosophila melanogaster) Unigene: 143080 Human Unigene: 247816 Human Unigene: 248172 Human Unigene: 248178 Human Unigene: 248179 Human Unigene: 278483 Human Unigene: 352191 Human Unigene: 46423 Human Unigene: 528055 Human Unigene: 533295 Human Unigene: 55468 Human Unigene: 591790 Human Unigene: 655235 Human Unigene: 662174 Human Unigene: 706635 Human Unigene: 742244 Human Unigene: 14775 Mouse Unigene: 158272 Mouse Unigene: 227295 Mouse Unigene: 228709 Mouse Unigene: 246720 Mouse Unigene: 255646 Mouse Unigene: 260530 Mouse Unigene: 261642 Mouse Unigene: 261662 Mouse Unigene: 261664 Mouse Unigene: 377875 Mouse Unigene: 442307 Mouse Unigene: 486099 Mouse Unigene: 489077 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
