| 產品編號 | bs-25205R |
| 英文名稱 | Rabbit Anti-RNASE4 antibody |
| 中文名稱 | 核糖核酸酶4抗體 |
| 別 名 | RAB1; Ribonuclease 4; Ribonuclease A B1; Ribonuclease, RNase A family, 4; RNAS4_HUMAN; RNase 4; RNS4. |
| 研究領域 | 腫瘤 心血管 細胞生物 信號轉導 血管內皮細胞 細胞骨架 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat (predicted: Mouse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:400-800,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 14kDa |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse RNASE4: 91-190/148 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene belongs to the pancreatic ribonuclease family. It plays an important role in mRNA cleavage and has marked specificity towards the 3' side of uridine nucleotides. Alternative splicing results in four transcript variants encoding the same protein. This gene and the gene that encodes angiogenin share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region. [provided by RefSeq, Aug 2013]. Function: This RNase has marked specificity towards the 3' side of uridine nucleotides. Subcellular Location: Secreted. Similarity: Belongs to the pancreatic ribonuclease family. SWISS: Q9JJH1 Gene ID: 58809 Database links: Entrez Gene: 465205 Chimpanzee Entrez Gene: 6038 Human Entrez Gene: 58809 Mouse Entrez Gene: 100174757 Orangutan Omim: 601030 Human SwissProt: Q8HZQ0 Chimpanzee SwissProt: P34096 Human SwissProt: Q9JJH1 Mouse SwissProt: Q5NVS4 Orangutan Unigene: 730830 Human Unigene: 202665 Mouse Unigene: 1742 Rat |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (RNASE4) Polyclonal Antibody, Unconjugated (bs-25205R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
