產品編號 | bsm-41489M |
英文名稱 | Mouse Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid antibody |
中文名稱 | SARS冠狀病毒2核衣殼蛋白單克隆抗體 |
別 名 | SARS-CoV-2 Nucleocapsid Protein; SARS-CoV-2 NP; nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2]; novel coronavirus N Protein; novel coronavirus Nucleocapsid Protein; 2019-nCoV Nucleoprotein; 2019-nCoV N; 2019nCoV N; 2019-nCoV N Protein; 2019 ncov N Protein; 2019-nCoV nucleocapsid protein. |
研究領域 | 細菌及病毒 |
抗體來源 | Mouse |
克隆類型 | Monoclonal |
克 隆 號 | 9G4 |
交叉反應 | (predicted: SARS-CoV-2) |
產品應用 | ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 46kDa |
性 狀 | Liquid |
濃 度 | >1mg/ml |
免 疫 原 | Recombinant SARS-CoV-2 Nucleocapsid Protein: 1-419/419 |
亞 型 | IgG2a |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M PBS (pH7.4) with 0.02% Proclin300. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產品介紹 |
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. SWISS: P0DTC9 Gene ID: 43740575 Database links: SARS-CoV-2 |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |