| 產品編號 | bsm-52254R |
| 英文名稱 | Rabbit Anti-GFAP antibody |
| 中文名稱 | 膠質纖維酸性蛋白重組兔單抗 |
| 別 名 | Astrocyte; FLJ45472; Glial Fibrillary Acidic Protein; Intermediate filament protein; GFAP_HUMAN. |
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Specific References (1) | bsm-52254R has been referenced in 1 publications.
[IF=4.755] Hou Yujun. et al. Maintenance of Intestinal Homeostasis in Diarrhea-Predominant Irritable Bowel Syndrome by Electroacupuncture Through Submucosal Enteric Glial Cell-Derived S-Nitrosoglutathione. FRONT PHYSIOL. 2022 Aug;0:1368 IF ; Rat.
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| 研究領域 | 腫瘤 細胞生物 免疫學 神經生物學 信號轉導 干細胞 細胞粘附分子 細胞類型標志物 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:2000-1:10000,IHC-P=1:500-1:1000,IHC-F=1:400-800,ICC/IF=1:50,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 48kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GFAP |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] Function: GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Subunit: Interacts with SYNM. Isoform 3 interacts with PSEN1 (via N-terminus). Subcellular Location: Cytoplasm. Note=Associated with intermediate filaments. Tissue Specificity: Expressed in cells lacking fibronectin. Post-translational modifications: Phosphorylated by PKN1. DISEASE: Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. Similarity: Belongs to the intermediate filament family. SWISS: P14136 Gene ID: 2670 星形膠質細胞標志物 （Astrocyte Marker） GFAP是一個56kDa的中間絲蛋白（intermediate filament，IF），在中樞神經系統發育期是一個特異性的標志物，以區別星形細胞和其它膠質細胞。GFAP表達在皮層和海馬,急、慢性皮質酮治療時表達減少。 GFAP可以和人、大鼠、小鼠的GFAP反應，在正常和腫瘤性的星形膠質細胞陽性表達，而神經節細胞、神經元、成纖維細胞、少突膠質細胞和這些細胞來源的腫瘤細胞陰性表達，主要用于星形膠質瘤等中樞神經系統腫瘤的診斷和鑒別診斷,GFAP的缺乏可導致AD病。 |
| 產品圖片 |
Sample:
Lane 1: Rat Cerebrum tissue lysates
Lane 2: Rat Cerebellum tissue lysates
Lane 3: Human U251 cell lysates
Primary: Anti-GFAP (bsm-52254R) at 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (bsm-52254R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (bsm-52254R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (MOUSE brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (bsm-52254R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (bsm-52254R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Cell line: Neuro-2a (Negative cell line)
Fixation: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary Ab incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: No staining on bsm-52254R
Cell line: Mouse primary astrocyte
Fixation: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary Ab incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-52254R
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
