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Rabbit Anti-Apoptosis Research Antibody Assembly kit  antibody (Abk001)  
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產品編號 Abk001
英文名稱 Rabbit Anti-Apoptosis Research Antibody Assembly kit  antibody
中文名稱 凋亡研究抗體套裝
別    名 Apoptosis Research Antibody Assembly kit(Bax,Caspase-3,Caspase-8,Caspase-9,Cytochrome C)  凋亡研究抗體套裝,凋亡抗體組合裝
研究領域 細胞生物  細胞凋亡  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,Mouse,Rat
產品應用 WB=1:500-2000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Apoptosis is the most common type of programmed cell death. The signal transduction of apoptosis involves the processing and activation of cysteinase caspase and the alternative mitochondrial pathway mediated by pro-apoptosis factors. Among them, the caspase mediating the initiation of apoptosis includes caspase-8,-9,-10 and -12, in which caspase-8 is mainly involved in tumor necrosis factor receptor (TNFR) superfamily death receptor mediated extrinsic apoptosis pathway. Caspase-3,-6 and -7 mediate downstream effects, including cleavage of cytoskeleton and chromatin, and eventually lead to apoptosis. In addition, mitochondria also play key roles in activation of apoptosis. Different upstream stimuli promote the binding of pro-apoptosis factors to the outer membrane of mitochondria through signal transduction, which leads to the change of mitochondrial permeability and the release of inner mitochondrial membrane (IMM) proteins (such as cytochrome C and other proteins). Cytochrome C coupling activation of caspase-9 can further activate key downstream effector caspases.
Apoptosis Research Antibody Assembly Kit is one economical package of selected antibodies for most representative apoptosis signaling pathway-related proteins, including caspase-8, Bax, cytochrome C, caspase-9 and caspase-3. These antibodies have been tested in a variety of applications and species. For specific application information of different antibodies, please refer to each individual antibody datasheet.

Apoptosis Research Antibody Assembly kit
Mouse Anti-Bax antibody (bsm-33279M)
Rabbit Anti-Caspase 3 precursor antibody (bs-2593R)
Rabbit Anti-Caspase-8 subunit p10 antibody (bs-20613R)
Rabbit Anti-Caspase-9 antibody (bs-20773R)
Rabbit Anti-Cytochrome C antibody (bsm-52050R)


SWISS:
N/A

Gene ID:
N/A

Database links:

Apoptosis Research Antibody Assembly kit

Mouse Anti-Bax antibody (bsm-33279M)

Rabbit Anti-Caspase 3 precursor antibody (bs-2593R)

Rabbit Anti-Caspase-8 subunit p10 antibody (bs-20613R)

Rabbit Anti-Caspase-9 antibody (bs-20773R)

Rabbit Anti-Cytochrome C antibody (bsm-52050R)



細胞凋亡是一種最常見的細胞程序性死亡。凋亡的信號傳導過程涉及半胱氨酸蛋白酶caspase 的加工和激活過程以及由促凋亡因子介導的線粒體旁路途徑。其中,介導凋亡起始的caspase包括caspase-8、-9、-10 和 -12,其中caspase-8主要參與由腫瘤壞死因子受體(TNFR)超家族死亡受體介導的外源性凋亡途徑。而介導下游效應的 caspase 包括 caspase-3、-6 和 -7,這些下游效應caspase 會導致細胞骨架蛋白和染色質被剪切并最終發生細胞凋亡。另外,凋亡還可以通過線粒體旁路途徑發生,一些不同的上游應激通過信號傳導過程促進促凋亡因子結合于線粒體外膜,導致線粒體通透性改變并釋放線粒體內膜蛋白(如細胞色素C等蛋白)。細胞色素C偶聯激活的 caspase-9,會進一步激活下游關鍵效應的 caspase并最終發生細胞凋亡。
凋亡研究抗體套裝極具性價比,包含了最具代表性的凋亡信號通路相關蛋白,包括介導外源性凋亡途徑的caspase-8、介導線粒體旁路途徑的促凋亡因子Bax和線粒體內膜蛋白細胞色素C、參與多條凋亡分支途徑的caspase-9以及介導下游效應的caspase-3。這些抗體在多種應用和物種中進行了測試。有關抗體具體使用信息,請參閱各個抗體產品數據表。
產品圖片
Primary: Anti-Caspase-9 (bs-20773R) at 1/300 dilution Sample: K562 Cell (Human) Lysate at 40 ug Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35/50 kD Observed band size: 35 kD
Primary: Anti-Caspase 3 precursor? (bs-2593R) at 1/1000 dilution Sample: Spleen (Mouse) Lysate at 40 ug Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28 kD
Primary: Anti-Bax (bsm-33279M) at 1/1000 dilution Sample: 293T(Human) Cell Lysate at 30 ug 293T-UV(Human) Cell Lysate at 30 ug Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 21 kD
Primary: Anti- Cytochrome C (bsm-52050R) at 1/1000 dilution Sample: Liver (Mouse) Lysate at 40 ug Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12.8/26 kD Observed band size: 12.8 kD
Primary: Anti- Caspase 8 (bs-20613R) at 1/300 dilution Sample: Thymus (Mouse) Lysate at 40 ug Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12,55 kD Observed band size: 17 kD
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