| 產品編號 | bs-17376R |
| 英文名稱 | Rabbit Anti-CBX4 antibody |
| 中文名稱 | 染色盒同源物4抗體 |
| 別 名 | CBX 4; CBX4_HUMAN; E3 SUMO-protein ligase CBX4; EC:2.3.2.-; Chromobox protein homolog 4; Polycomb 2 homolog (Pc2; hPc2); Pc2; hPc2; |
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Specific References (3) | bs-17376R has been referenced in 3 publications.
[IF=4.658] Hu C et al. CBX4 promotes the proliferation and metastasis via regulating BMI‐1 in lung cancer. J Cell Mol Med. 2019 Nov 13. WB&IHC-P ; Human&Mouse.
[IF=4.011] Xu J et al. Regulatory effect of two Trichinella spiralis serine protease inhibitors on the host's immune system. Sci Rep. 2019 Nov 19;9(1):17045. WB ; Mouse.
[IF=1.91] Han, Xiao, et al. "HIF-1α SUMOylation affects the stability and transcriptional activity of HIF-1α in human lens epithelial cells." Graefe's Archive for Clinical and Experimental Ophthalmology 253.8 (2015): 1279-1290. WB ; Human.
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| 研究領域 | 細胞生物 結合蛋白 表觀遺傳學 泛素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Pig,Sheep,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 61kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CBX4: 401-500/560 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Enables SUMO binding activity; SUMO ligase activity; and enzyme binding activity. Involved in negative regulation of transcription by RNA polymerase II. Located in nuclear body. Part of PRC1 complex. Implicated in hepatocellular carcinoma. Biomarker of hepatocellular carcinoma. [provided by Alliance of Genome Resources, Apr 2022] Function: E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Subunit: Interacts with histone H3-K9Me3. Interacts with CHTOP (By similarity). Component of a PRC1-like complex. Self-associates. Interacts with SUV39H1 and HIPK2. Interacts with CSNK2B. Subcellular Location: Nucleus. Tissue Specificity: Ubiquitous. Post-translational modifications: Phosphorylated on Thr-497 by HIPK2 upon DNA damage; which enhances E3 SUMO-protein ligase activity and promotes sumoylation on Lys-494. Similarity: Contains 1 chromo domain. SWISS: O00257 Gene ID: 8535 Database links: Entrez Gene: 8535 Human Entrez Gene: 12418 Mouse SwissProt: O00257 Human SwissProt: O55187 Mouse |
| 產品圖片 |
Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Rat Liver tissue lysates
Lane 3: Human K562 cell lysates
Lane 4: Human HL-60 cell lysates
Lane 5: Human HeLa cell lysates
Lane 6: Human HepG2 cell lysates
Lane 7: Human HUVEC cell lysates
Lane 8: Human A549 cell lysates
Primary: Anti-CBX4 (bs-17376R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (CBX4) polyclonal Antibody, Unconjugated (bs-17376R) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
