| 產品編號 | bs-14663R |
| 英文名稱 | Rabbit Anti-EXOC6B antibody |
| 中文名稱 | EXOC6B蛋白抗體 |
| 別 名 | EXC6B_HUMAN; EXOC6B; Exocyst complex component 6B; Exocyst complex component Sec15B; KIAA0919; SEC15-like protein 2; SEC15B; SEC15L2. |
| 研究領域 | 細胞生物 信號轉導 轉運蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Rat (predicted: Mouse,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 94kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EXOC6B: 721-811/811 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
In yeast and rat, Sec15 is part of a multiprotein complex that is required for targeted exocytosis.[supplied by OMIM, Apr 2004] Function: Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. Subunit: The exocyst complex is composed of SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70 and EXO84. Similarity: Belongs to the SEC15 family. SWISS: Q9Y2D4 Gene ID: 23233 Database links: Entrez Gene: 23233 Human Entrez Gene: 75914 Mouse Omim: 607880 Human SwissProt: Q9Y2D4 Human SwissProt: A6H5Z3 Mouse Unigene: 303454 Human Unigene: 159621 Mouse |
| 產品圖片 |
Sample:Hela (Human)Cell Lysate at 40 ug
Primary: Anti-EXOC6B(bs-14663R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 94kD
Observed band size: 94kD
Paraformaldehyde-fixed, paraffin embedded (Rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EXOC6B) Polyclonal Antibody, Unconjugated (bs-14663R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EXOC6B) Polyclonal Antibody, Unconjugated (bs-14663R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
