| 產品編號 | bs-10170R |
| 英文名稱 | Rabbit Anti-FLT3L antibody |
| 中文名稱 | FMS樣酪氨酸激酶3配體抗體 |
| 別 名 | Flt 3 ligand; Flt 3L; Flt3 L; FLT3 LG; Flt3 ligand; Flt3L; FLT3L_HUMAN; FLT3LG; Fms related tyrosine kinase 3 ligand; Fms-related tyrosine kinase 3 ligand; SL cytokine. |
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Specific References (1) | bs-10170R has been referenced in 1 publications.
[IF=3.816] Lun Yao. et al. Recombinant Pseudorabies Virus with TK/gE Gene Deletion and Flt3L Co-Expression Enhances the Innate and Adaptive Immune Response via Activating Dendritic Cells. Viruses-Basel. 2021 Apr;13(4):691 WB,IF ; Pig.
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| 研究領域 | 心血管 細胞生物 干細胞 細胞分化 淋巴細胞 t-淋巴細胞 b-淋巴細胞 細胞因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Pig,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 23kDa |
| 細胞定位 | 細胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FLT3L: 61-160/235 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Flt 3 ligand (Flt 3-L), variously designated Flt 3/Flk 2 ligand or FL, is a hematopoietic growth factor that stimulates the proliferation of stem and CD34+ progenitor cells and has been cloned from both mouse and human genomes. Flt 3-L is a potent in vitro growth stimulator of granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3), and G-CSF-dependent granulocyte-macrophage committed precursors from Lin CD34+ bone marrow cells as well as other primitive B cell populations. Additionally, Flt 3-L stimulates the proliferation of hematopoietic progenitor cells isolated from mouse fetal liver or adult mouse bone marrow. Flt 3-L does not, however, affect the growth of erythroid-committed progenitors. Flt 3-L exists in two forms and is active as both a soluble and as a membrane-bound ligand. The Flt 3-L receptor, Flt 3, is a tyrosine kinase expressed on CD34+ cells that shares a high degree of homology with the SCF (stem cell factor) receptor, c-Kit and c-Fms. Function: Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. Subunit: Homodimer (isoform 2). Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. SWISS: P49771 Gene ID: 2323 Database links: Entrez Gene: 2323 Human Entrez Gene: 719239 Rhesus monkey Omim: 600007 Human SwissProt: P49771 Human Unigene: 428 Human
Flt3L是一種可以促進多種干細胞、血細胞及其前體細胞生成、分化的細胞因子,對T淋巴細胞、B淋巴細胞和NK細胞和DC的前體細胞都有促進增生、誘導分化的功能.Flt3L可與其它促細胞生長的細胞因子有很好的協同作用.目前,尚未發現具有毒性作用,是一種良好的擴增劑,對研究細胞的形態、細胞表現型和功能具有重要的意義. |
| 產品圖片 |
Sample: intestine (Mouse) Lysate at 40 ug
Primary: Anti-FLT3L(bs-10196R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 28 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
