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Rabbit Anti-FENS1  antibody (bs-13169R)  
~~~促銷代碼KT202411~~~
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產品編號 bs-13169R
英文名稱 Rabbit Anti-FENS1  antibody
中文名稱 磷酸肌醇結合蛋白1抗體
別    名 FENS 1; FENS-1; KIAA1435; Phosphoinositide binding protein 1; Phosphoinositide binding protein SR1; Phosphoinositide-binding protein 1; WD repeat and FYVE domain containing 1; WD repeat and FYVE domain-containing protein 1; WD40 and FYVE domain containing protein 1; WD40- and FYVE domain-containing protein 1; WDF1; WDFY1; WDFY1_HUMAN; ZFYVE17; Zinc finger FYVE domain containing protein 17; Zinc finger FYVE domain-containing protein 17.  
Specific References  (2)     |     bs-13169R has been referenced in 2 publications.
[IF=7.561] Alexandru Tatomir. et al. RGC-32 Acts as a Hub to Regulate the Transcriptomic Changes Associated With Astrocyte Development and Reactive Astrocytosis. Front Immunol. 2021; 12: 705308  IHC ;  Mouse.  
[IF=3.921] Anna Sancho-Balsells et al. Lack of Helios During Neural Development Induces Adult Schizophrenia-Like Behaviors Associated With Aberrant Levels of the TRIF-Recruiter Protein WDFY1. Front Cell Neurosci. 2020 May 14;14:93.  WB ;  Mouse.  
研究領域 細胞生物  信號轉導  轉運蛋白  結合蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Dog,Horse)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 46kDa
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human FENS1: 21-120/410 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 WD-repeats are motifs that are found in a variety of proteins and are characterized by a conserved core of 40-60 amino acids that commonly form a tertiary propeller structure. While proteins that contain WD-repeats participate in a wide range of cellular functions, they are generally involved in regulatory mechanisms concerning chromatin assembly, cell cycle control, signal transduction, RNA processing, apoptosis and vesicular trafficking. WDFY1 (WD repeat and FYVE domain containing 1), also known as WDF1, FENS-1 or ZFYVE17, is a 410 amino acid protein that localizes to the early endosome and contains one FYVE-type zinc finger and seven WD repeats through which it may play a role in protein trafficking and signal transduction.

Subunit:
Binds PtdIns3P in vitro with high specificity over other phosphoinositides.

Subcellular Location:
Early endosome.

Similarity:
Contains 1 FYVE-type zinc finger.
Contains 7 WD repeats.

SWISS:
Q8IWB7

Gene ID:
57590

Database links:

Entrez Gene: 57590 Human

Entrez Gene: 69368 Mouse

Entrez Gene: 301549 Rat

SwissProt: Q8IWB7 Human

Unigene: 368359 Human

Unigene: 8557 Rat



產品圖片
Sample: Lane 1: Cerebrum (Mouse) Tissue Lysate at 40 ug Lane 2: Cerebrum (Rat) Tissue Lysate at 40 ug Lane 3: Skin (Rat) Tissue Lysate at 40 ug Lane 4: Huvec (Human) Cell Lysate at 30 ug Lane 5: HepG2 (Human) Cell Lysate at 30 ug Lane 6: THP-1 (Human) Cell Lysate at 30 ug Primary: Anti-FENS1 (bs-13169R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 48 kD
Sample: Lane 1: U87MG (Human) Cell Lysate at 30 ug Lane 2: HepG2 (Human) Cell Lysate at 30 ug Lane 3: U2os (Human) Cell Lysate at 30 ug Lane 4: A431 (Human) Cell Lysate at 30 ug Primary: Anti-FENS1 (bs-13169R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 46 kD
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FENS1) Polyclonal Antibody, Unconjugated (bs-13169R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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