| 產品編號 | bs-11198R |
| 英文名稱 | Rabbit Anti-ProSAPiP1 antibody |
| 中文名稱 | 含脯氨酸突觸相關蛋白相互作用蛋白1抗體 |
| 別 名 | KIAA0552; PRIP1_HUMAN; Proline rich synapse associated protein interacting protein 1; ProSAP-interacting protein 1; ProSAPiP1; ProSAPiP1 protein. |
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Specific References (1) | bs-11198R has been referenced in 1 publications.
[IF=2.624] Liyang Tong. et al. Topical bendazol inhibits experimental myopia progression and decreases the ocular accumulation of HIF‐1α protein in young rabbits. Ophthal Physl Opt. 2020 Sep;40(5):567-576 WB ; Rat.
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| 研究領域 | 神經生物學 細胞粘附分子 細胞膜蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,Flow-Cyt=1μg/Test,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 72kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ProSAPiP1 : 151-250/673 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The ProSAP family of proteins contain many protein-protein interaction domains and serve as scaffolding mediators within the post-synaptic density (PSD) of excitatory brain synapses. The PSD is an electron-dense structure underneath the post-synaptic plasma membrane of excitatory synapses that anchors and clusters glutamate receptors opposite to the pre-synaptic neurotransmitter release site. Shank proteins contain PDZ modular domains that coordinate the synaptic localization of ion channels, receptors, signaling enzymes, and cell adhesion molecules. The PDZ domain mediates protein-protein interactions via the recognition of a conserved sequence motif at the C-terminus of their target protein(s). ProSAPiP1 (proline rich synapse associated protein interacting protein 1) is a 673 amino acid protein that interacts with the PDZ domain of Shank 3. ProSAPiP1 expression is brain-specific with highest expression within the cerebellum, hippocampus and cerebral cortex. Subunit: Interacts (via C-terminus) with SHANK3 (via PDZ domain). Interacts (via coiled coil) with SIPA1L1. Can form homooligomers (By similarity). Subcellular Location: Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cytoplasm > cytoskeleton. Detected at synapses, postsynaptic density, synaptic spines and dendrites. Similarity: Belongs to the PROSAPIP1 family. SWISS: O60299 Gene ID: 9762 Database links: Entrez Gene: 9762 Human Omim: 610484 Human SwissProt: O60299 Human Unigene: 90232 Human |
| 產品圖片 |
Sample:
Eye (Mouse) Lysate at 40 ug
Primary: Anti- ProSAPiP1 (bs-11198R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 72 kD
Observed band size: 72 kD
Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice..
Isotype Control Antibody: Rabbit IgG(orange) ;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue),
Dilution: 1:200 in 1 X PBS containing 0.5% BSA ;
Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
