| 產品編號 | bs-11107R |
| 英文名稱 | Rabbit Anti-PCDH11X antibody |
| 中文名稱 | 原鈣粘附蛋白11X抗體 |
| 別 名 | PCDH11; PCDH22; PCDHX; PCDHY; Protocadherin 11; Protocadherin 11 X linked; Protocadherin 11 Y linked; Protocadherin 22; Protocadherin on the X chromosome; Protocadherin on the Y chromosome; Protocadherin PC; Protocadherin prostate cancer; PC11X_HUMAN. |
| 研究領域 | 腫瘤 神經生物學 細胞粘附分子 細胞骨架 細胞膜蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Rabbit,Pig,Sheep,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 145kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PCDH11X: 601-700/1347 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. The encoded protein consists of an extracellular domain containing 7 cadherin repeats, a transmembrane domain and a cytoplasmic tail that differs from those of the classical cadherins. The gene is located in a major X/Y block of homology and its Y homolog, despite divergence leading to coding region changes, is the most closely related cadherin family member. The protein is thought to play a fundamental role in cell-cell recognition essential for the segmental development and function of the central nervous system. Transcripts arising from alternative splicing encode isoforms with variable cytoplasmic domains. [provided by RefSeq, Jul 2008]. Function: Potential calcium-dependent cell-adhesion protein. Subcellular Location: Cell membrane; Single-pass type I membrane protein (Potential). Tissue Specificity: Expressed strongly in fetal brain and brain (cortex, amygdala, thalamus, substantia nigra, hippocampus, caudate nucleus and corpus callosum). Expressed at low level in testis. Similarity: Contains 7 cadherin domains. SWISS: Q9BZA7 Gene ID: 27328 Database links: Entrez Gene: 27328 Human Entrez Gene: 245578 Mouse Omim: 300246 Human SwissProt: Q9BZA7 Human |
| 產品圖片 |
Sample:
Lane 1: Human SH-SY5Y cell lysates
Lane 2: Human U251 cell lysates
Lane 3: Human U87MG cell lysates
Primary: Anti- PCDH11X (bs-11107R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 145 kDa
Observed band size: 120 kDa
Sample:
Lane 1: Mouse Hippocampus tissue lysates
Lane 2: Mouse Cerebral cortex tissue lysates
Primary: Anti-PCDH11X (bs-11107R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 145 kDa
Observed band size: 110 kDa
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
