| 產品編號 | bs-11095R |
| 英文名稱 | Rabbit Anti-NEGR1 antibody |
| 中文名稱 | 神經生長調節蛋白1抗體 |
| 別 名 | A kindred of IgLON; DMML2433; IgLON family member 4; IGLON4; KILON; Neuronal growth regulator 1; Neurotractin; Ntra; PRO4993; UNQ2433; NEGR1_HUMAN. |
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Specific References (1) | bs-11095R has been referenced in 1 publications.
[IF=6.064] Haofuzi Zhang. et al. Identification of a Fibroblast-Related Prognostic Model in Glioma Based on Bioinformatics Methods. BIOMOLECULES. 2022 Nov;12(11):1598 IHC ; Human.
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| 研究領域 | 發育生物學 神經生物學 細胞粘附分子 細胞膜蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 31kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NEGR1: 38-130/354 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
NEGR1 is a 354 amino acid protein belonging to the IgLON family and immunoglobulin superfamily. NEGR1 may play a role in cell adhesion and regenerative axon sprouting in the mammalian brain and is highly expressed in adult hippocampus, cerebrum and brainstem, with much lower levels found in cerebellum. Localizing to the cell membrane at the glycosylphosphatidylinositol anchor (GPI) anchor, NEGR1 contains three Ig-like C2-type (immunoglobulin-like) domains. NEGR1 is encoded by a gene that maps to human chromosome 1p31.1, and is one of several loci associated with body mass index (BMI), possibly contributing to the development of obesity. Function: NEGR1 may be involved in cell-adhesion. May function as a trans-neural growth-promoting factor in regenerative axon sprouting in the mammalian brain. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor Similarity: Belongs to the immunoglobulin superfamily. IgLON family. Contains 3 Ig-like C2-type (immunoglobulin-like) domains. SWISS: Q7Z3B1 Gene ID: 257194 Database links: Entrez Gene: 257194 Human Entrez Gene: 320840 Mouse Omim: 613173 Human SwissProt: Q7Z3B1 Human SwissProt: D3Z4T6 Mouse |
| 產品圖片 |
Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-NEGR1 (bs-11095R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 37 kD
Sample:
Brain (Mouse) Lysate at 40 ug
Primary: Anti-NEGR1 (bs-11095R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 37 kD
Sample:
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-NEGR1 (bs-11095R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 37 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
