| 產品編號 | bs-9432R |
| 英文名稱 | Rabbit Anti-GERP antibody |
| 中文名稱 | 環指蛋白27抗體 |
| 別 名 | RNF27; Lioblastoma expressed ring finger protein; Ring finger protein 27; RNF27; RP11-47A8.4; TRIM8; Tripartite motif containing 8; Tripartite motif protein TRIM8; TRIM8_HUMAN. |
| 研究領域 | 腫瘤 細胞生物 鋅指蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Sheep,Chicken,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 61kDa |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GERP/TRIM8/RNF27: 61-160/551 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The tripartite motif (TRIM) family of proteins are characterized by a conserved TRIM domain that includes a coiled-coil region, a B-box type zinc finger, one RING finger and three zinc-binding domains. TRIM8 (tripartite motif containing 8), also known as GERP (glioblastoma-expressed RING finger protein) or RNF27 (RING finger protein 27), is a 551 amino acid protein that is thought to function as an E3 ubiquitin-protein ligase that promotes SOCS-1 proteasomal degradation. As a widely expressed homodimer, TRIM8 localizes to nuclear bodies and contains two B box-type zinc fingers and one RING-type zinc finger. TRIM8 is expressed in lung, heart, brain and skeletal muscle, with low levels detected in intestine, placenta, leukocytes and liver. The gene encoding TRIM8 maps to human chromosome 10q24.32. Function: Probable E3 ubiquitin-protein ligase which may promote proteasomal degradation of SOCS1. Subunit: Homodimer. Interacts with SOCS1 (via) SH2 domain and SOCS box. Tissue Specificity: Widely expressed. Similarity: Belongs to the TRIM/RBCC family. Contains 2 B box-type zinc fingers. Contains 1 RING-type zinc finger. SWISS: Q9BZR9 Gene ID: 81603 Database links: Entrez Gene: 81603 Human Entrez Gene: 93679 Mouse Omim: 606125 Human SwissProt: Q9BZR9 Human SwissProt: Q99PJ2 Mouse Unigene: 336810 Human Unigene: 392177 Mouse Unigene: 481672 Mouse 葛博：bs-9432R 細胞定位：細胞質 細胞核 2017.11.08日張鳳英修改 |
| 產品圖片 |
Sample:
Placenta (Mouse) Lysate at 40 ug
Primary: Anti- GERP (bs-9432R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61kD
Observed band size: 61kD
Sample:
HepG2 Cell (Human) Lysate at 30 ug
Hela Cell(Human)Lysate at 30 ug
Primary: Anti- GERP (bs-9432R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61kD
Observed band size: 61kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
