| 產品編號 | bs-0327R-HRP |
| 英文名稱 | Rabbit Anti-Bovine IgM antibody |
| 中文名稱 | 辣根過氧化物酶標記的兔抗牛IgM |
| 別 名 | Rabbit Anti-Bovine IgM (HRP); Immunoglobulin M; |
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Specific References (2) | bs-0327R-HRP has been referenced in 2 publications.
[IF=3.471] Tongsheng Qi. et al. Seroepidemiology of Neosporosis in Various Animals in the Qinghai-Tibetan Plateau. FRONT VET SCI. 2022 Jul 19;9:953380 ELISA ; Neospora caninum.
[IF=3.231] Jinchao Zhang. et al. Serological Analysis of IgG and IgM Antibodies against Anaplasma spp. in Various Animal Species of the Qinghai-Tibetan Plateau. ANIMALS. 2022 Jan;12(19):2723 ELISA ; Bovine.
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| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Bovine |
| 產品應用 | WB=1:1000-10000,IHC-P=1:100-500,IHC-F=1:100-1000,ELISA=1:1000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性 狀 | Liquid |
| 濃 度 | 2.0 mg/ml |
| 免 疫 原 | Native Bovine IgM |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存條件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品介紹 |
IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
