| 產品編號 | bs-7552R |
| 英文名稱 | Biglycan Rabbit pAb |
| 中文名稱 | 骨/軟骨蛋白多糖1抗體 |
| 別 名 | BGN; Biglycan proteoglycan; Bone/cartilage proteoglycan I; Dermatan sulphate proteoglycan I; DSPG1; PG S1; PG-S1; PGI; PGS1_HUMAN; SLRR1A; Small leucine rich protein 1A. |
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Specific References (4) | bs-7552R has been referenced in 4 publications.
[IF=8.554] Jin Wang. et al. TLR4 aggravates microglial pyroptosis by promoting DDX3X-mediated NLRP3 inflammasome activation via JAK2/STAT1 pathway after spinal cord injury. CLIN TRANSL MED. 2022 Jun;12(6):e894 IP, WB ; Mouse.
[IF=5.23] Han, Chao, et al. "An Animal Model of Modic Changes by Embedding Autogenous Nucleus Pulposus inside Subchondral Bone of Lumbar Vertebrae." Scientific Reports 6 (2016): 35102. WB ; Rabbit.
[IF=2.36] Sim?es et al. Small leucine-rich proteoglycans (SLRPs) in the endometrium of polycystic ovary syndrome women: a pilot study. (2017) J.Ovarian.Res. 10:54 IHC ; Human.
[IF=1.72] Huang, Guo-fu, et al. "The Effect of Electroacupuncture on the Extracellular Matrix Synthesis and Degradation in a Rabbit Model of Disc Degeneration." Evidence-Based Complementary and Alternative Medicine 2014 (2014). IHC-P ; Rabbit.
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| 研究領域 | 細胞生物 免疫學 信號轉導 干細胞 細胞骨架 細胞外基質 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 37 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞外基質 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Biglycan: 101-200/368 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
May be involved in collagen fiber assembly. Tissue specificity; Found in several connective tissues, especially in articular cartilages. Function: May be involved in collagen fiber assembly (By similarity). Subunit: Homodimer. Forms a ternary complex with MFAP2 and ELN Subcellular Location: Secreted, extracellular space, extracellular. Tissue Specificity: Found in several connective tissues, especially in articular cartilages. Post-translational modifications: The two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate (By similarity). Similarity: Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. Contains 12 LRR (leucine-rich) repeats. SWISS: P21810 Gene ID: 633 Database links: Entrez Gene: 633 Human Entrez Gene: 12111 Mouse Omim: 301870 Human SwissProt: P21810 Human SwissProt: P28653 Mouse Unigene: 821 Human Unigene: 2608 Mouse Unigene: 783 Rat |
| 產品圖片 |
Sample: Bone (mouse) Lysate at 40 ug
Primary: Anti- Biglycan (bs-7552R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37kD
Observed band size: 52 kD
Sample:
Colon (Rat) Lysate at 40 ug
Embryo (Mouse) Lysate at 40 ug
Primary: Anti-Biglycan (bs-7552R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 37 kD
Observed band size: 52 kD
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Biglycan Polyclonal Antibody, Unconjugated(bs-7552R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Biglycan) Polyclonal Antibody, Unconjugated (bs-7552R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
