| 產品編號 | bs-6073R |
| 英文名稱 | MFSD2A Rabbit pAb |
| 中文名稱 | 促進調解蛋白家族2A抗體 |
| 別 名 | Major facilitator superfamily domain containing 2; Major facilitator superfamily domain containing 2A; Major facilitator superfamily domain-containing protein 2A; MFS2A_HUMAN; MFSD2; MFSD2A. |
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Specific References (2) | bs-6073R has been referenced in 2 publications.
[IF=5.168] Díaz-Carballo et al. Cytotoxic stress induces transfer of mitochondria-associated human endogenous retroviral RNA and proteins between cancer cells. (2017) Oncotarget. 8:95945-95964 FCM ; Human.
[IF=1.862] Xiaohui Chang. et al. Imatinib reduces the fertility of male mice by penetrating the blood-testis barrier and inducing spermatogonia apoptosis. Reprod Biol. 2021 Sep;21:100527 WB,IHC ; Mouse.
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| 研究領域 | 腫瘤 神經生物學 信號轉導 轉錄調節因子 G蛋白偶聯受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=0.2ug/test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 60 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MFSD2A: 331-430/543 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
MFSD2 is a 543 amino acid multi-pass membrane protein of the endoplasmic reticulum that is involved in beta-adrenergic signaling during thermogenesis. Existing as three alternatively spliced isoforms, MFSD2 plays a role in G1 regulation and is encoded by a gene that maps to human chromosome 1p34.2. Human chromosome 1 spans 260 million base pairs, contains over 3,000 genes, comprises nearly 8% of the human genome and houses a large number of disease-associated genes, including those that are involved in familial adenomatous polyposis, Stickler syndrome, Parkinson’s disease, Gaucher disease, schizophrenia and Usher syndrome. Function: Play a role in thermogenesis via beta-adrenergic signaling pathway. Subcellular Location: Endoplasmic reticulum membrane. Tissue Specificity: Similarity: Belongs to the major facilitator superfamily. SWISS: Q8NA29 Gene ID: 84879 Database links: Entrez Gene: 84879 Human Entrez Gene: 76574 Mouse Omim: 614397 Human SwissProt: Q8NA29 Human SwissProt: Q9DA75 Mouse Unigene: 655177 Human Unigene: 331842 Mouse |
| 產品圖片 |
Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-MFSD2A (bs-6073R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 60/65 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat se
Blank control:Molt-4.
Primary Antibody (green line): Rabbit Anti-MFSD2A antibody (bs-6073)
Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 0.2μg /test.
Protoco
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
