| 產品編號 | C-0006 |
| 英文名稱 | Normal Rabbit Serum |
| 中文名稱 | 封閉用正常兔血清（原液） |
| 別 名 | Normal Rabbit serum. Whole serum is obtained from non-immunized (preimmune) healthy Rabbits. The serum is clarified through a 0.45micron low protein binding filter. 兔血清; 兔血清封閉液; 正常血清; 動物非免疫血清（兔）; |
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Specific References (3) | C-0006 has been referenced in 3 publications.
[IF=6.6] Lin Liu. et al. Giardia duodenalis and Its Secreted PPIB Trigger Inflammasome Activation and Pyroptosis in Macrophages through TLR4-Induced ROS Signaling and A20-Mediated NLRP3 Deubiquitination. Cells-Basel. 2021 Dec;10(12):3425 IF ; Mouse.
[IF=3.448] Yong Y et al. Electroacupuncture pretreatment attenuates brain injury in a mouse model of cardiac arrest and cardiopulmonary resuscitation via the AKT/eNOS pathway. Life Sci. 2019 Aug 30;235:116821. Other ;
[IF=2.425] Xie W et al. Seasonal expressions of prolactin, prolactin receptor and STAT5 in the scented glands of the male muskrats (Ondatra zibethicus). Eur J Histochem. 2019 Jan 17;63(1). IHC-P ;
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| 保存條件 | Store at -20℃. |
| 產品介紹 |
Rabbit Serum blocking reagent can be used as a blocking agent to treat plastic surfaces, membrane or tissue after they have been sensitized with primary antibody or antigen. It provides an alternative to bovine serum albumin (BSA) and non-fat dry milk. It is effective in reducing nonspecific binding of proteins to reaction surfaces, thereby maximizing signal-to-noise ratio. This blocking agent is recommended for use in immunoassays where the primary antibody was produced in goat, as a source of non-specific serum protein or on tissue for immunohistochemical applications. Application: Rabbit Serum blocking reagent is ideal for blocking procedures such as Western Blotting, ELISA and immunochemistry to prevent nonspecific binding. 封閉血清適用于免疫組化或者免疫熒光實驗中樣本的封閉。可以封閉待檢樣本中與蛋白質非特異性結合的位點，另外還可以封閉樣品中內源性的 Fc 片段結合位點，阻斷抗體與樣品中的 Fc 受體結合，從而降低背景，減少假陽性。在選擇封閉血清時，要注意封閉用血清中不能含有目標蛋白，且與一抗來源不同，可用與二抗相同來源的封閉血清。正常兔血清被廣泛應用于組織和細胞染色中非特異性抗體結合的阻斷，以及抗體的其他應用。血清可直接用于阻斷，或作為阻斷緩沖液的成分。 使用方法 免疫組化： 用5-10%正常兔血清封閉（1:10-1:20倍稀釋），室溫孵育5-10分鐘。清除血清，勿洗，滴加適當比例稀釋的一抗或一抗工作液，37℃或室溫孵育4℃過夜。 細胞涂片： 用5-10%正常兔血清封閉（1:10-1:20倍稀釋），使用 PBS 稀釋（ 0.01mol/L pH7.4），少量點滴：漠過細胞涂片即可. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
