產(chǎn)品編號(hào) | bs-2222R |
英文名稱 | Rabbit Anti-Endoglucanase 25 antibody |
中文名稱 | 內(nèi)切葡聚糖酶25抗體 |
別 名 | GUN25_ARATH; Endoglucanase 25; Cellulase homolog OR16pep; Endo-1,4-beta glucanase 25; Protein KORRIGAN; Protein RADIALLY SWOLLEN 2. 內(nèi)切葡聚糖酶25; 纖維素酶同源物OR16pep; |
研究領(lǐng)域 | 藥物及化合物 |
抗體來(lái)源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | (predicted: Arabidopsis Thaliana) |
產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 69kDa |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from Arabidopsis thaliana Endoglucanase 25: 101-200/621 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
Cellulase is any of several enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis, the decomposition of cellulose and of some related polysaccharides. Cellulases break down the cellulose molecule into monosaccharides ("simple sugars") such as beta-glucose, or shorter polysaccharides and oligosaccharides. Cellulose breakdown is of considerable economic importance, because it makes a major constituent of plants available for consumption and use in chemical reactions. The specific reaction involved is the hydrolysis of the 1,4-beta-D-glycosidic linkages in cellulose, hemicellulose, lichenin, and cereal beta-D-glucans. Because cellulose molecules bind strongly to each other, cellulolysis is relatively difficult compared to the breakdown of other polysaccharides such as starch. Function: Required for cellulose microfibrils formation. Involved in cell wall assembly during cell elongation and cell plate maturation in cytokinesis. Required for secondary cell wall formation in the developing xylem. May cycle through different intracellular compartments, including plasma membrane. Subcellular Location: Cell membrane; Single-pass type II membrane protein. Note=Cell plate. Tissue Specificity: Highly expressed in roots and stems, at intermediate levels in leaves and flowers, and at lower levels in siliques. Expressed in xylem (at protein level). Post-translational modifications: Glycosylated. N-glycosylation of KOR in the endoplasmic reticulum followed by N-glycan modifications in the Golgi are essential for catalytic activity. Similarity: Belongs to the glycosyl hydrolase 9 (cellulase E) family. SWISS: Q38890 Gene ID: 835035 Database links:
纖維素酶(cellulase) 它是與脫落直接有關(guān)的酶,菜豆、棉花和柑橘葉片脫落時(shí),纖維素酶活性增加。從菜豆葉柄離區(qū)中分離出pI9.5和pI4.5(分別稱為9.5或4.5纖維素酶)兩種纖維素酶,前者與細(xì)胞壁木質(zhì)化有關(guān),受IAA調(diào)控;后者與細(xì)胞壁降解有關(guān),受乙烯調(diào)控。測(cè)定柑橘小葉片離區(qū)的各個(gè)不同區(qū)段中的纖維素酶活性,發(fā)現(xiàn)酶活性最高的部位是在離區(qū)的近軸端(靠近莖的0.22mm處),所以纖維素酶的活性不一定與離層細(xì)胞的分開(kāi)直接有關(guān),而可能與離層分離后的保護(hù)層發(fā)育的關(guān)系更為直接。 |