| 產品編號 | bs-2418R |
| 英文名稱 | Rabbit Anti-NCR3 antibody |
| 中文名稱 | 細胞毒性受體NK-p30（CD337）抗體 |
| 別 名 | Natural cytotoxicity triggering receptor 3; 1C7; Activating natural killer receptor p30; Activating NK A1 receptor; CD337; CD337 antigen; LY117; Lymphocyte antigen 117; MALS; Natural cytotoxicity triggering receptor 3; Natural cytotoxicity triggering receptor 3; Natural killer cell p30 related protein; Natural killer cell p30 related protein; Natural killer cell p30-related protein; NK-p30; NKp30; NCTR3_HUMAN. |
| 研究領域 | 免疫學 干細胞 細胞表面分子 自然殺傷細胞 淋巴細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Rat,Rabbit,Dog,Horse) |
| 產品應用 | Flow-Cyt=2ug/Test,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 20kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD337: 81-180/201 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
NCR3 is a cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. It is a member of the Ig superfamily and may cooperate with NKp46 and NKp44 in the induction of cytotoxicity against a variety of target cells and is selectively expressed by all NK cells, both freshly isolated and cultured in IL2, thus representing an optimal marker for NK cell identification. Similar to NKp46 and CD16, NKp30 associated with CD3 zeta chains becomes tyrosine phosphorylated following cell treatment with pervanadate. Its extracelluar portion is characterized by a single domain of the V-type and by a region rich in hydrophobic amino acids, potentially involved in protein/protein interactions that connects the Ig V-like domain with the transmembrane portion. It is selectively expressed by all resting and activated NK cells and weakly expressed in spleen. Function: Cytotoxicity-activating receptor that contributes to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. Engagement of NCR3 by BAG6 also promotes dendritic cell (DC) maturation, both through killing those DCs that did not properly acquire a mature phenotype, and inducing NK cells to release TNFA and IFNG, which promotes DC maturation. Subunit: Homodimer in the unliganted form. Interacts with CD3Z. Interacts with and is activated by binding to B7H6. Interacts with and is activated by binding to BAG6. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Selectively expressed by all resting and activated NK cells and weakly expressed in spleen. Similarity: Belongs to the natural cytotoxicity receptor (NCR) family. Contains 1 Ig-like (immunoglobulin-like) domain. SWISS: O14931 Gene ID: 259197 Database links: Entrez Gene: 259197 Human Omim: 611550 Human SwissProt: O14931 Human Unigene: 509513 Human Unigene: 203206 Rat 細胞毒性受體家族（NCRs）主要有：NKp46、NKp44和NKp30，其中NKp46和NKp30表達于所有靜止和活化的NK細胞，而NKp44則選擇性的表達于活化的NK細胞。靶細胞上的配體分子與NCRs的結合（交聯）引發NK細胞的活化導致靶細胞的裂解和大量細胞因子的產生?，F已確認NKp46和NKp44可以識別病毒上的配體分子紅血球凝聚素，而NKp46則能識別腫瘤細胞上的乙酰硫酸多聚糖。 |
| 產品圖片 |
Blank control: HL60.
Primary Antibody (green line): Rabbit Anti-NCR3 antibody (bs-2418R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Black line : Positive blank control (HL60); Negative blank control (HUVEC）
Green line : Primary Antibody (Rabbit Anti-NCR3 antibody (bs-2418R) )
Orange line：Isotype Control Antibody (Rabbit IgG) .
Blue line : Secondary Antibody (Goat anti-rabbit IgG-AF647)
HL60（Positive）and HUVEC（Negative control）cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with NCR3 Antibody(bs-2418R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
