| 產品編號 | bs-1570R |
| 英文名稱 | Rabbit Anti-APOH antibody |
| 中文名稱 | 載脂蛋白H抗體 |
| 別 名 | Apolipoprotein H; Activated protein C binding protein; APC inhibitor; APOH; B2G1; beta 2 glycoprotein I; beta 2 glycoprotein I precursor; BG; OTTMUSP00000003033; APOH_HUMAN. |
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Specific References (2) | bs-1570R has been referenced in 2 publications.
[IF=38.104] Du Xing. et al. B cell-derived anti-beta 2 glycoprotein I antibody mediates hyperhomocysteinemia-aggravated hypertensive glomerular lesions by triggering ferroptosis. SIGNAL TRANSDUCT TAR. 2023 Mar;8(1):1-16 Other ; Mouse.
[IF=4.543] Subhojit Paul. et al. Modulation of lung cytoskeletal remodeling, RXR based metabolic cascades and inflammation to achieve redox homeostasis during extended exposures to lowered pO 2. 2021 May 17 WB ; Human, Rat.
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| 研究領域 | 心血管 免疫學 合成與降解 糖蛋白 脂蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 36kDa |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ApoH: 281-345/345 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Apolipoprotein H is expressed by placental trophoblast cells at high levels. Although the normal physiological role is not known, the protein appears to act as a co-factor for the binding of autoantibodies to phospholipids to trophoblasts, which is a process involved in the pathogenesis of recurrent miscarriage. Function: Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells. Subcellular Location: Secreted. Tissue Specificity: Expressed by the liver and secreted in plasma. Post-translational modifications: N- and O-glycosylated. PubMed:6587378 also reports glycosylation on 'Asn-188' for their allele. Similarity: Contains 4 Sushi (CCP/SCR) domains. SWISS: P02749 Gene ID: 350 Database links: Entrez Gene: 350 Human Omim: 138700 Human SwissProt: P02749 Human Unigene: 445358 Human Unigene: 1824 Rat 載脂蛋白H(apolipoprotein D,apoH)又稱為β2糖蛋白Ⅰ(β2-glycoprotein,β2-GI)，是最初是在人血漿的高密度脂蛋白中被分離出來的。apoH在結構上與其他類型的載脂蛋白存在很大的差異,被歸入脂肪促成素家族。 |
| 產品圖片 |
Sample:
HepG2 Cell (Human) Lysate at 30 ug
Primary: Anti-APOH (bs- 1570R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 35/50 kD
Sample:
A549 Cell (Human) Lysate at 30 ug
Primary: Anti-APOH (bs- 1570R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 35/50 kD
Sample:
Lane 1: Liver (Mouse) Lysate at 40 ug
Lane 2: Liver (Rat) Lysate at 40 ug
Lane 3: Pancreas (Rat) Lysate at 40 ug
Primary: Anti-APOH (bs-1570R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44-50 kD
Observed band size: 47 kD
Tissue/cell: human laryngeal cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-APOH Polyclonal Antibody, Unconjugated(bs-1570R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
