| 產品編號 | bs-0088R |
| 英文名稱 | Rabbit Anti-Secretin antibody |
| 中文名稱 | 分泌素抗體 |
| 別 名 | SCT; Secretin precursor; SECR_HUMAN; Secr. |
 |
Specific References (5) | bs-0088R has been referenced in 5 publications.
[IF=14.957] Yuchu ?Liu. et al. A gut-brain axis mediates sodium appetite via gastrointestinal peptide regulation on a medulla-hypothalamic circuit. SCI ADV. 2023 Feb;9(7) IF ; Mouse.
[IF=14.679] Lixian Chen. et al. Inhibition of secretin/secretin receptor axis ameliorates non‐alcoholic fatty liver disease phenotypes. 2021 Apr 30 IHC ; Human, Mouse.
[IF=9.071] Lamei Xue. et al. Maternal secretin ameliorates obesity by promoting white adipose tissue browning in offspring. EMBO REP. 2022 Jul;23(7):e54132 IHC ; Mouse.
[IF=6.4] Zhang et al. The knockout of secretin in cerebellar Purkinje cells impairs mouse motor coordination and motor learning. (2014) Neuropsychopharmacolog. 39:1460-8 IHC-P ; Mouse.
[IF=0.392] Yildiz, M.. et al. The Effect of Chronic Intermittent Cold Exposure on Gastrin-, Somatostatin-, Secretin-, and Serotonin-Containing Cells in the Small Intestine of Rats. Biol Bull+. 2021 Dec;48(3):S95-S106 IHC ; Rat.
|
| 研究領域 | 信號轉導 生長因子和激素 內分泌病 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Rat (predicted: Mouse,Pig,Goat) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 13kDa |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Secretin |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Secretin belongs to the glucagon family. This protein is an endocrine hormone and its major site of production is the endocrine S cells located in the proximal small intestinal mucosa. The release of active secretin is stimulated by either fatty acids or an acidic pH in the duodenum. This hormone stimulates the secretion of bicarbonate-rich pancreatic fluids and has also been shown to regulate the growth and development of the stomach, small intestine, and pancreas. Secretin deficiency has been implicated in autistic syndrome, suggesting that the hormone could have a neuroendocrine function in addition to its role in digestion. Function: Stimulates formation of NaHCO(3)-rich pancreatic juice and secretion of NaHCO(3)-rich bile and inhibits HCl production by the stomach. Subcellular Location: Secreted. Similarity: Belongs to the glucagon family. SWISS: P09683 Gene ID: 6343 Database links: Entrez Gene: 6343 Human Omim: 182099 Human SwissProt: P09683 Human Unigene: 632324 Human Secretin 促胰液素或胰泌素，由十二指腸分泌的一種多肽激素，可刺激胰液的釋放。 |
| 產品圖片 |
Sample:
Lane 11: Human SW480 cell lysates
Lane 12: Human MCF-7 cell lysates
Lane 13: Human HepG2 cell lysates
Primary: Anti-Secretin (bs-0088R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13 kDa
Observed band size: 13 kDa
Tissue/cell: rat intestine tissue ; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Secrein Polyclonal Antibody, Unconjugated(bs-0088R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
