| 產品編號 | bs-0062R |
| 英文名稱 | CALB2 Rabbit pAb |
| 中文名稱 | 鈣結合蛋白抗體 |
| 別 名 | CALB2_HUMAN; Calretinin; CR; 29 kDa calbindin; CAB29; |
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Specific References (1) | bs-0062R has been referenced in 1 publications.
[IF=3.9] Suzui, Masumi, et al. "Multiwalled carbon nanotubes intratracheally instilled into the rat lung induce development of pleural malignant mesothelioma and lung tumors." Cancer Science 107.7 (2016): 924-935. IHC-P ; Rat.
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| 研究領域 | 神經生物學 結合蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Pig,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 29 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Calretinin: 211-271/271 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Calretinin is a calcium-binding protein which is abundant in auditory neurons. It belongs to the calbindin family. Calbindin 2 (calretinin), closely related to calbindin 1, is an intracellular calcium-binding protein belonging to the troponin C superfamily. Calbindin 1 is known to be involved in the vitamin-D-dependent calcium absorption through intestinal and renal epithelia, while the function of neuronal calbindin 1 and calbindin2 is poorly understood. The sequence of the calbindin 2 cDNA reveals an open reading frame of 271 codons coding for a protein of 31,520 Da, and shares 58% identical residues with human calbindin1. Function: Calretinin is a calcium-binding protein which is abundant in auditory neurons. Tissue Specificity: Brain. Similarity: Belongs to the calbindin family. Contains 6 EF-hand domains. SWISS: P22676 Gene ID: 794 Database links: Entrez Gene: 396255 Chicken Entrez Gene: 794 Human Entrez Gene: 12308 Mouse Entrez Gene: 393684 Zebrafish Omim: 114051 Human SwissProt: P07090 Chicken SwissProt: P22676 Human SwissProt: Q08331 Mouse Unigene: 106857 Human Unigene: 2755 Mouse Unigene: 10321 Rat 鈣結合蛋白(Calretinin,CR)主要存在于神經組織中，近10%的腺癌可以表達此蛋白的局部染色陽性，而絕大多數的間皮瘤為陽性表達，因此它是診斷間皮瘤的一個有用的標記物 。 鈣結合蛋白是平滑肌和非肌性調控蛋白。它可以與肌動蛋白、肌球蛋白、原肌球蛋白和鈣調素相互作用。 |
| 產品圖片 |
Sample:
Lane 1: Mouse Cerebrum Lysates
Lane 2: Mouse Cerebellum Lysates
Lane 3: Rat Cerebrum Lysates
Primary: Anti- Calretinin (bs-0062R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kDa
Ob
Sample:
U-87MG(Human) Cell Lysate at 30 ug
Primary: Anti-Calretinin (bs-0062R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 29 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat se
Blank control:A431.
Primary Antibody (green line): Rabbit Anti-S100A13 antibody (bs-2617R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
