| 產(chǎn)品編號 | bs-0094R |
| 英文名稱 | Rabbit Anti-AACT antibody |
| 中文名稱 | α-1抗胰糜蛋白酶抗體 |
| 別 名 | AACT_HUMAN; SERPINA3; Alpha-1-antichymotrypsin; ACT; Cell growth-inhibiting gene 24/25 protein; Serpin A3; GIG24; GIG25; serpin family A member 3; |
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Specific References (1) | bs-0094R has been referenced in 1 publications.
[IF=3.13] Wang, Robert YL, et al. "Proteome Demonstration of Alpha-1-Acid Glycoprotein and Alpha-1-Antichymotrypsin." Pediatric Infectious Disease Journal (2014). WB ; Human.
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| 研究領(lǐng)域 | 心血管 細(xì)胞生物 神經(jīng)生物學(xué) 糖尿病 泛素 Alzheimer's |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 45kDa |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human AACT: 121-320/423 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Alpha 1-Antichymotrypsin, a member of the serine proteinase inhibitor (serpin) family, inhibits neutrophil proteinase cathepsin G and mast cell chymases, and protects the lower respiratory tract from damage by proteolytic enzymes. It contains a reactive centre loop, which interacts with cognate proteinases, resulting in loop cleavage and a major conformational change. Recently, alpha 1-antichymotrypsin has been identified as a major constituent of the neurofibrillary plaques associated with Alzheimers disease, and in vitro studies have shown that it enhances the rate of amyloid-fibril formation. These observations and recent genetic evidence suggest that alpha 1-antichymotrypsin is important in the pathogenesis of Alzheimers disease. Function: Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2. Subunit: Interacts with DNAJC1. Subcellular Location: Secreted. Tissue Specificity: Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains. Similarity: Belongs to the serpin family. SWISS: P01011 Gene ID: 12 Database links: Entrez Gene: 12 Human Omim: 107280 Human SwissProt: P01011 Human Unigene: 534293 Human Unigene: 710488 Human AACT是由神經(jīng)膠質(zhì)細(xì)胞分泌并參與淀粉樣纖維生成和沉積的一種色氨酸蛋白酶抑制劑,被認(rèn)為是Aβ肽的假性底物，與Aβ肽具有很高的親合性，可促進(jìn)Aβ肽的纖維化以生成淀粉樣沉積。 ApoE作為AD的另一種病理伴隨性物質(zhì)，也可識別并與Aβ肽相結(jié)合，促進(jìn)Aβ肽沉積生成淀粉樣老年斑塊。由于AACT和ApoE在AD發(fā)病中具有共同的作用途徑，因此探討其相互作用可能是AD治療中一個(gè)新的途徑。 AACT是一種絲氨酸蛋白酶抑制劑，可以中和糜蛋白酶等酶活性的蛋白酶，存在于大多數(shù)的組織細(xì)胞、巨噬細(xì)胞以及多種胃腸道和肺部腫瘤中，但多形核白細(xì)胞中無此物質(zhì)，它可以作為組織細(xì)胞瘤和良性/惡性纖維組織細(xì)胞瘤的標(biāo)志。此抗體與人的AACT反應(yīng)，主要用于惡性纖維組織細(xì)胞瘤等惡性腫瘤的診斷,也可用于胃癌﹑肺癌、腎癌等腫瘤的研究。 |
| 產(chǎn)品圖片 |
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-Alpha-1-Antichymotrypsin (bs-0094R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 47 kD
Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Rat Liver tissue lysates
Primary: Anti-Alpha-1-Antichymotrypsin (bs-0094R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Tissue/cell: Mouse liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti- Alpha-1-Antichymotrypsin Polyclonal Antibody, Unconjugated (bs-0094R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti- Alpha-1-Antichymotrypsin Polyclonal Antibody, Unconjugated (bs-0094R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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