產品編號 | bs-13775R-Bio |
英文名稱1 | Rabbit Anti-PSMD12/Biotin Conjugated antibody |
中文名稱 | 生物素標記的26S蛋白酶調節亞型p55抗體 |
別 名 | 26S Proteasome regulatory subunit p55; 26S proteasome non-ATPase regulatory subunit 12; 26S proteasome regulatory subunit p55; 26S proteasome regulatory subunit RPN5; MGC75406; p55; proteasome (prosome, macropain) 26S subunit, non ATPase12; Proteasome 26S non ATPase subunit 12 isoform 2; PSD12_HUMAN; PSMD12; Rpn5. |
規格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul |
研究領域 | 細胞生物 信號轉導 泛素 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | |
產品應用 | WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 52kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human PSMD12 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產品介紹 |
background: In eukaryotic cells, selective breakdown of cellular proteins is ensured by their ubiquitination and subsequent degradation by the 26S Proteasome. The 26S Proteasome is a protease complex that selectively breaks down proteins that have been modified by polyubiquitin chains. It is made up of two multisubunit complexes: the 20S Proteasome chamber, which serves as the proteolytic core of the complex and two 19S regulatory particles which recognize and unfold ubiquitinated proteins. PSMD12 (proteasome (prosome, macropain) 26S subunit, non-ATPase, 12), also known as p55 or Rpn5, is a 456 amino acid protein belonging to the proteasome subunit p55 family. PSMD12 acts as a regulatory subunit of the 26S proteasome and is a component of the PA700 complex. PSMD12 contains one PCI domain. Function: Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Similarity: Belongs to the proteasome subunit p55 family. Contains 1 PCI domain. Database links: Entrez Gene: 5718 Human Entrez Gene: 66997 Mouse Omim: 604450 Human SwissProt: O00232 Human SwissProt: Q9D8W5 Mouse Unigene: 592689 Human Unigene: 21667 Mouse Unigene: 15873 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |