| 產品編號 | bs-0778R-PE |
| 英文名稱1 | Rabbit Anti-CD90/PE Conjugated antibody |
| 中文名稱 | PE標記的CD90抗體 |
| 別 名 | CD90 / Thy1; CD7; CD90; CD90 antigen; CDw90; FLJ33325; MGC128895; T25; Theta antigen; Thy-1; Thy 1; Thy 1 cell surface antigen; Thy 1 membrane glycoprotein; Thy 1 membrane glycoprotein precursor; Thy 1.2; Thy-1 T-cell antigen; Thy1 antigen; Thy1 T cell antigen; Thy1.1; Thy1.2; Thymus cell antigen 1, theta; THY1_RAT; THY1_HUMAN. |
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Journal
PMID
IF
Application
[IF=4.122] Wang?C et al. Chaihulonggumulitang Shows Psycho-cardiology Therapeutic Effects on Acute Myocardial Infarction by Enhancing Bone Marrow Mesenchymal Stem Cells Mobilization. Sci Rep.?2018 Feb 27;8(1):3724. FCM ; Rat.
[IF=3.989] Zhang C et al. CXCR4-Overexpressing Umbilical Cord Mesenchymal Stem Cells Enhance Protection against Radiation-Induced Lung Injury. Stem Cells Int. 2019 Feb 5;2019:2457082. FCM ; Human.
[IF=3.73] Di Tomo, Pamela, et al. "Calcium Sensing Receptor Expression in Ovine Amniotic Fluid Mesenchymal Stem Cells and the Potential Role of R-568 during Osteogenic Differentiation."?PloS one?8.9 (2013): e73816. Sheep.
[IF=3.08] Zhan, Fu‐Liang, Xin‐Yang Liu, and Xing‐Bo Wang. "The Role of MicroRNA‐143‐5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway." Journal of Cellular Biochemistry (2017). FCM ; Human.
[IF=2.959] Zhan et al. The Role of MicroRNA-143-5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway. (2018) J.Cell.Biochem. 119:536-546 FC/FACS ; Human.
[IF=1.448] Gao Y et al. Induction of autophagy protects human dental pulp cells from lipopolysaccharide?induced pyroptotic cell death. Exp Ther Med. 2020 Mar;19(3):2202-2210. FCM ; Human.
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| 規格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 細胞生物 免疫學 神經生物學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, Rat, (predicted: Dog, Pig, Horse, Sheep, ) |
| 產品應用 | Flow-Cyt=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 12kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from rat Thy-1 (52-100aa) |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: Thy-1 or CD90 (Cluster of Differentiation 90) is a 25–37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. Thy-1can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 lead to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to some of the initial biochemical description and characterization of a vertebrate GPI anchor and also the first demonstration of tissue specific differential glycosylation. Function: May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Tissue Specificity: Abundant in lymphoid tissues. Post-translational modifications: Glycosylation is tissue specific. Sialylation of N-glycans at Asn-93 in brain and at Asn-42, Asn-93 and Asn-117 in thymus. Similarity: Contains 1 Ig-like V-type (immunoglobulin-like) domain. Database links: Entrez Gene: 7070 Human Entrez Gene: 21838 Mouse Omim: 188230 Human SwissProt: P04216 Human SwissProt: P01831 Mouse Unigene: 644697 Human Unigene: 3951 Mouse Unigene: 108198 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品圖片 |
Blank control: Mouse kidney.
Primary Antibody (green line): Rabbit Anti-CD90 antibody (bs-0778R-PE)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
