| 產品編號 | bsm-52078R |
| 英文名稱 | Rabbit Anti-FOXP1 antibody |
| 中文名稱 | 叉頭蛋白P1重組兔單抗 |
| 別 名 | FLJ23741; Fork head related protein like B; Forkhead box P1; Forkhead box protein P1; 12CC4; foxp1; FOXP1_MOUSE; FOX P1; FOXP 1; Glutamine rich factor 1; hFKH1B; HSPC215; MGC12942; MGC88572; MGC99551; QRF 1; QRF1. |
| 研究領域 | 腫瘤 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 15E2 |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:1000-2000,IHC-P=1:100-500,IHC-F=1:50-200),IF=1:50-200)
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 79kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human FOXP1 protein, around C-terminal 150aa |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene belongs to subfamily P of the forkhead box (FOX) transcription factor family. Forkhead box transcription factors play important roles in the regulation of tissue- and cell type-specific gene transcription during both development and adulthood. Forkhead box P1 protein contains both DNA-binding- and protein-protein binding-domains. This gene may act as a tumor suppressor as it is lost in several tumor types and maps to a chromosomal region (3p14.1) reported to contain a tumor suppressor gene(s). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] Function: Transcriptional repressor. It plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B cell development. Subunit: Forms homodimers and heterodimers with FOXP2 and FOXP4. Dimerization is required for DNA-binding. Interacts with CTBP1. Subcellular Location: Nucleus. Tissue Specificity: Highest expression in the lung, brain, and spleen. Lower expression in heart, skeletal muscle, kidney, small intestine (isoform 3 not present) and liver. Similarity: Contains 1 C2H2-type zinc finger. Contains 1 fork-head DNA-binding domain. SWISS: Q9H334 Gene ID: 27086 Database links: Entrez Gene: 27086 Human Entrez Gene: 108655 Mouse SwissProt: Q9H334 Human SwissProt: P58462 Mouse |
| 產品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at
room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L
(HRP)
Lysate: 1: HeLa, 2: MCF-7, 3: Daudi
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 75kDa
Observed MW: 50-95 kDa
Tissue: rat colon
Section type: Formalin fixed & Paraffin -
embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at
room temperature
Secondary ab: Anti-Rabbit and Mouse Polymer HRP
Counter stain:
Hematoxylin (Blue)
Comment: Color brown is the positive signal
for bsm-52078R
Tissue: mouse colon
Section type: Formalin fixed & Paraffin -
embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at
room temperature
Secondary ab: Anti-Rabbit and Mouse Polymer HRP
Counter stain:
Hematoxylin (Blue)
Comment: Color brown is the positive signal
for bsm-52078R
Tissue: human colon
Section type: Formalin fixed & Paraffin -
embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at
room temperature
Secondary ab: Anti-Rabbit and Mouse Polymer HRP
Counter stain:
Hematoxylin (Blue)
Comment: Color brown is the positive signal
for bsm-52078R
Tissue: human colon carcinoma
Section type: Formalin fixed & Paraffin -
embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at
room temperature
Secondary ab: Anti-Rabbit and Mouse Polymer HRP
Counter stain:
Hematoxylin (Blue)
Comment: Color brown is the positive signal
for bsm-52078R
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
