| 產品編號 | bs-23618R |
| 英文名稱 | Rabbit Anti-TAS1R3 antibody |
| 中文名稱 | G蛋白偶聯受體TAS1R3抗體 |
| 別 名 | TS1R3_HUMAN; Taste receptor type 1 member 3; Sweet taste receptor T1R3; T1R3, TR3; |
| 研究領域 | 細胞生物 免疫學 神經生物學 信號轉導 G蛋白偶聯受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 92kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TAS1R3: 431-530/852 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene is a G-protein coupled receptor involved in taste responses. The encoded protein can form a heterodimeric receptor with TAS1R1 to elicit the umami taste response, or it can bind with TAS1R2 to form a receptor for the sweet taste response. [provided by RefSeq, Nov 2015] Function: Putative taste receptor. TAS1R1/TAS1R3 responds to the umami taste stimulus (the taste of monosodium glutamate). TAS1R2/TAS1R3 recognizes diverse natural and synthetic sweeteners. TAS1R3 is essential for the recognition and response to the disaccharide trehalose (By similarity). Sequence differences within and between species can significantly influence the selectivity and specificity of taste responses. Subunit: Forms homodimers or heterodimers with TAS1R1 and TAS1R2. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Expressed in vascular endothelium. Post-translational modifications: Phosphorylated by FER and other protein-tyrosine kinases. Phosphorylated at Ser-288 by PAK7/PAK5. Dephosphorylated by PTPRJ. Similarity: Belongs to the G-protein coupled receptor 3 family. TAS1R subfamily. SWISS: Q7RTX0 Gene ID: 83756 Database links: Entrez Gene: 83756 Human Omim: 605865 Human SwissProt: Q7RTX0 Human Unigene: 74375 Human |
| 產品圖片 |
Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human HeLa cell lysates
Lane 3: Human HL-60 cell lysates
Lane 4: Human K562 cell lysates
Lane 5: Human Jurkat cell lysates
Primary: Anti-TAS1R3 (bs-23618R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 92 kDa
Observed band size: 120 kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
