產品編號 | bsm-51155M |
英文名稱 | Mouse Anti-FASN antibody |
中文名稱 | 脂肪酸合成酶單克隆抗體 |
別 名 | fatty acid synthase; FASN; FAS; FA Synthase 1; MGC14367; MGC15706; OA-519; Fatty acid synthase; [Acyl-carrier-protein] S-acetyltransferase; [Acyl-carrier-protein] S-malonyltransferase; 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase; 3-oxoacyl-[acyl-carrier-protein] reductase; 3-oxoacyl-[acyl-carrier-protein] synthase; Enoyl-[acyl-carrier-protein] reductase; Oleoyl-[acyl-carrier-protein] hydrolase; FAS_MHUMAN. |
Specific References (3) | bsm-51155M has been referenced in 3 publications.
[IF=3.654] Yingjun Zhou. et al. Chrysin improves diabetic nephropathy by regulating the AMPK-mediated lipid metabolism in HFD/STZ-induced DN mice. J FOOD BIOCHEM. 2022 Aug;:e14379 WB ; Mouse.
[IF=3.448] Yang X et al. miR‐760 exerts an antioncogenic effect in esophageal squamous cell carcinoma by negatively driving fat metabolism via targeting c‐Myc. J Cell Biochem. 2019 Nov 10. WB ; Human.
[IF=3.231] Jing Fan. et al. Chitosan Oligosaccharide Inhibits the Synthesis of Milk Fat in Bovine Mammary Epithelial Cells through AMPK-Mediated Downstream Signaling Pathway. ANIMALS. 2022 Jan;12(13):1692 WB ; Bovine.
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研究領域 | 腫瘤 心血管 免疫學 神經生物學 信號轉導 合成與降解 |
抗體來源 | Mouse |
克隆類型 | Monoclonal |
克 隆 號 | 2C5 |
交叉反應 | Human (predicted: Mouse) |
產品應用 | WB=1:500-5000,IHC-P=1:100-500,IHC-F=1:20-200,ICC/IF=1:100-500,IF=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 274kDa |
細胞定位 | 細胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human FASN |
亞 型 | IgM |
純化方法 | affinity purified by Protein AGL |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產品介紹 |
FASN (Fatty Acid Synthase) is a central enzyme in de novo lipogenesis. FAS is a target for SREBP and is upregulated by LXR activation; it is also one of the accepted markers for insulin resistance, SREBP and LXR activation. Function: Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein. Subunit: Homodimer which is arranged in a head to tail fashion. Subcellular Location: Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Tissue Specificity: Ubiquitous. Prominent expression in brain, lung, and liver. Similarity: Contains 1 acyl carrier domain. SWISS: P49327 Gene ID: 2194 Database links: Entrez Gene: 2194 Human Entrez Gene: 14104 Mouse Omim: 600212 Human SwissProt: P12276 Chicken SwissProt: P49327 Human SwissProt: P19096 Mouse Unigene: 8951 Chicken Unigene: 30099 Cow Unigene: 83190 Human Unigene: 236443 Mouse Unigene: 9486 Rat |
產品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with FASN monoclonal antibody, unconjugated (bsm-51155M) at 1:4000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (FASN) Monoclonal Antibody, Unconjugated (bsm-51155M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (FASN) Monoclonal Antibody, Unconjugated (bsm-51155M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (FASN) Monoclonal Antibody, Unconjugated (bsm-51155M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (FASN) Monoclonal Antibody, Unconjugated (bsm-51155M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |