| 產品編號 | bs-20750R |
| 英文名稱 | Rabbit Anti-UCP-2 antibody |
| 中文名稱 | 線粒體脫偶連蛋白2抗體 |
| 別 名 | Uncoupling Protein-2; UCP2; UCP 2; BMIQ4; Mitochondrial uncoupling protein 2; SLC25A8; UCPH; Uncoupling protein 2; Uncoupling protein 2 mitochondrial proton carrier; Solute carrier family 25 member 8; UCP2_HUMAN. |
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Specific References (3) | bs-20750R has been referenced in 3 publications.
[IF=7.129] Li Xu. et al. Fenpropathrin increases gliquidone absorption via causing damage to the integrity of intestinal barrier. ECOTOX ENVIRON SAFE. 2022 Sep;242:113882 WB ; Rat.
[IF=6.312] Jianwei Wang. et al. A Preliminary Study on the Effect of Hydrogen Gas on Alleviating Early CCl4-Induced Chronic Liver Injury in Rats. Antioxidants-Basel. 2021 Dec;10(12):1933 WB ; Rat.
[IF=5.279] Lei Wang. et al. Lactobacillus plantarum DP189 Reduces α-SYN Aggravation in MPTP-Induced Parkinson’s Disease Mice via Regulating Oxidative Damage, Inflammation, and Gut Microbiota Disorder. J Agr Food Chem. 2022;70(4):1163–1173 WB ; Mouse.
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| 研究領域 | 細胞生物 免疫學 染色質和核信號 糖尿病 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 34kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human UCP-2: 121-220/309 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. UCP2 gene is expressed in many tissues, with the greatest expression in skeletal muscle. UCP2 is thought to play a role in non shivering thermogenesis, obesity and diabetes. Function: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Subunit: Acts as a dimer forming a proton channel (By similarity). Subcellular Location: Mitochondrion inner membrane; Multi-pass membrane protein. Tissue Specificity: Widely expressed in adult human tissues, including tissues rich in macrophages. Most expressed in white adipose tissue and skeletal muscle. Similarity: Belongs to the mitochondrial carrier family. Contains 3 Solcar repeats. SWISS: P55851 Gene ID: 7351 Database links: Entrez Gene: 7351 Human Omim: 601693 Human SwissProt: P55851 Human Unigene: 80658 Human Unigene: 13333 Rat UCP-2又稱解耦聯蛋白 2 (UCP2 )是 1997年發現的一種新的解偶聯蛋白 ,它是線粒體內膜載體家族的一員 。與其類似物UCP1相比 ,UCP2在棕色脂肪組織中含量較低 ,但在體內分布廣泛。解耦聯蛋白(UCP)具有使線粒體呼吸鏈解耦聯作用,解耦聯所釋放的能量以熱能的形式散發。在對UCP與產熱關系的研究中,發現UCP2具有調節機體能量代謝的功能,并且可能是肥胖與2型糖尿病相關聯的一個重要基因。 |
| 產品圖片 |
Sample:
Lane 1: Stomach (Mouse) Lysate at 40 ug
Lane 2: Spleen (Mouse) Lysate at 40 ug
Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 4: Du145 (Human) Cell Lysate at 30 ug
Primary:
Anti-UCP-2 (bs-20750R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD
Sample:
Hela(Human) Cell Lysate at 30 ug
A673(Human) Cell Lysate at 30 ug
Primary: Anti-UCP-2 (bs-20750R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (UCP-2) Polyclonal Antibody, Unconjugated (bs-20750R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
