| 產品編號 | bs-20452R |
| 英文名稱 | Rabbit Anti-DLX2 antibody |
| 中文名稱 | 同源轉錄因子DLX2抗體 |
| 別 名 | DII A; Distal less homeo box 2; Distal less homeobox 2; Distal-less homeobox 2; DLX 2; Dlx2; DLX2_HUMAN; Dlx5; Homeobox protein DLX 2; Homeobox protein DLX-2; Homeobox protein DLX2; Homeobox protein TES 1; Homeobox protein TES-1; Homeobox protein TES1; Tes 1; Tes-1; Tes1; zgc. |
| 研究領域 | 細胞生物 神經生物學 信號轉導 干細胞 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 34kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DLX2: 51-150/328 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Dlx genes are a highly conserved family of six different (Dlx1–6) homeo box-containing genes that share homology with distal-less (Dll), a gene expressed in the head and limbs of the developing fruit fly. Dlx genes are expressed in spatially and temporally restricted patterns in craniofacial primordia, basal telencephalon and diencephalon, and in distal regions of extending appendages, including the limb and the genital bud. The differential expression of Dlx influences patterning, morphogenesis and histogenesis in these tissues. The Dlx gene products can activate transcription and are localized primarily to the nucleus, although Dlx-5 can be found in the cytoplasm. Dlx proteins influence different stages of proper tissue development, including patterning of the orofacial skeleton (craniofacial ectomesenchyme) and differentiation of structures within and between teeth. Function: Likely to play a regulatory role in the development of the ventral forebrain. May play a role in craniofacial patterning and morphogenesis. Subcellular Location: Nucleus. Similarity: Belongs to the distal-less homeobox family. Contains 1 homeobox DNA-binding domain. SWISS: Q07687 Gene ID: 1746 Database links: Entrez Gene: 1746 Human Entrez Gene: 13392 Mouse Omim: 126255 Human SwissProt: Q07687 Human SwissProt: P40764 Mouse Unigene: 419 Human Unigene: 3896 Mouse |
| 產品圖片 |
Sample:293T (Human)Cell Lysate at 40 ug
Primary: Anti-DLX2(bs-20452R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 34kD
Observed band size: 34kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DLX2) Polyclonal Antibody, Unconjugated (bs-20452R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DLX2) Polyclonal Antibody, Unconjugated (bs-20452R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
