產(chǎn)品編號(hào) | bs-21109R |
英文名稱(chēng) | Rabbit Anti-SFRS15 antibody |
中文名稱(chēng) | 富含精氨酸/絲氨酸剪切因子15抗體 |
別 名 | CTD-binding SR-like protein RA4; DKFZp434E098; FLJ23364; KIAA1172; Pre mRNA splicing SR protein rA4; SCAF4; SFR15_HUMAN; Splicing factor arginine/serine rich 15; Splicing factor serine alanine 15; SR like CTD associated factor 4; SR-related and CTD-associated factor 4; SRA4. |
研究領(lǐng)域 | 細(xì)胞生物 表觀遺傳學(xué) |
抗體來(lái)源 | Rabbit |
克隆類(lèi)型 | Polyclonal |
交叉反應(yīng) | Human,Mouse (predicted: Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 126kDa |
細(xì)胞定位 | 細(xì)胞核 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human SFRS15: 501-600/1147 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
This gene likely encodes a member of the arginine/serine-rich splicing factor family. A similar protein in Rat appears to bind the large subunit of RNA polymerase II and provide a link between transcription and pre-mRNA splicing. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2009] Function: May act to physically and functionally link transcription and pre-mRNA processing. Subcellular Location: Nucleus. Similarity: Belongs to the splicing factor SR family. Contains 1 CID domain. Contains 1 RRM (RNA recognition motif) domain. SWISS: O95104 Gene ID: 57466 Database links: Entrez Gene: 57466 Human Entrez Gene: 224432 Mouse SwissProt: O95104 Human Unigene: 17255 Human Unigene: 439811 Mouse |
產(chǎn)品圖片 |
Sample:
Thymus (Mouse) Lysate at 40 ug
K562(Human) Cell Lysate at 30 ug
HL60(Human) Cell Lysate at 30 ug
SIHA(Human) Cell Lysate at 30 ug
Primary: Anti- SFRS15 (bs-21109R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 126 kD
Observed band size: 126 kD
Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti-SFRS15 (bs-21109R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 126 kD
Observed band size: 126 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SFRS15) Polyclonal Antibody, Unconjugated (bs-21109R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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