| 產品編號 | bs-20185R |
| 英文名稱 | Rabbit Anti-POFUT1 antibody |
| 中文名稱 | 蛋白O巖藻糖基轉移酶1抗體 |
| 別 名 | FUT 12; FUT12; GDP fucose protein O fucosyltransferase 1; GDP-fucose protein O-fucosyltransferase 1; O Fuc T; o fucosyltransferase protein; O FucT 1; O FucT; O FucT1; O FUT; O-FucT-1; OFUT 1; OFUT; OFUT1; OFUT1_HUMAN; Peptide O fucosyltransferase1; Peptide-O-fucosyltransferase 1; Pofut 1; POFUT1; Protein O fucosyltransferase1. |
| 研究領域 | 腫瘤 細胞生物 信號轉導 干細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 41kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human POFUT1: 161-260/388 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Glycosyltransferases that mediate the regio- and stereoselective transfer of sugars, such as the fucosyltransferases, determine cell surface-carbohydrate profiles, which is an essential interface for biological recognition processes. Fucosyltransferases catalyze the covalent association of fucose to different positional linkages in sugar acceptor molecules. POFUT1 (protein O-fucosyltransferase 1), also known as FUT12, O-FUT or O-FucT-1, is a 388 amino acid protein that localizes to the endoplasmic reticulum and belongs to the fucosyltransferase subfamily of glycosyltransferases. Highly expressed in pancreas, kidney, lung, heart, brain, liver, placenta and skeletal muscle, POFUT1 uses manganese to catalyze the attachment (specifically the O-glycosidic linkage) of fucose to a conserved serine or threonine residue on a protein acceptor. Via its cataytic activity, POFUT1 plays an important role in Notch signaling, as Notch ligands can serve as POFUT1 substrates. Two isoforms of POFUT1 exist due to alternative splicing events. Function: Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains. Plays a crucial role in Notch signaling. Subcellular Location: Endoplasmic reticulum. Tissue Specificity: Highly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Post-translational modifications: N-glycosylated. Similarity: Belongs to the glycosyltransferase 68 family. SWISS: Q9H488 Gene ID: 23509 Database links: Entrez Gene: 23509 Human Entrez Gene: 140484 Mouse Omim: 607491 Human SwissProt: Q9H488 Human SwissProt: Q91ZW2 Mouse Unigene: 472409 Human Unigene: 293761 Mouse Unigene: 214685 Rat |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
