| 產品編號 | bs-12848R |
| 英文名稱 | Rabbit Anti-SURF5 antibody |
| 中文名稱 | 過量位點蛋白5抗體 |
| 別 名 | MED22; MED22_HUMAN; MED24; Mediator complex subunit 22; Mediator of RNA polymerase II transcription subunit 22; OTTHUMP00000022480; OTTMUSP00000012503; RGD1564893; Surf 5; Surf-5; Surfeit 5; Surfeit locus protein 5. |
| 研究領域 | 細胞生物 轉錄調節因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human,Mouse,Rat,Sheep,Cow,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 22kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SURF5: 1-100/200 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a protein component of the mediator complex, which functions in the regulation of transcription by bridging interactions between gene-specific regulatory factors, RNA polymerase II, and general transcription factors. Alternatively spliced transcript variants encoding different isoforms have been observed. [provided by RefSeq, Jul 2013] Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Subcellular Location: Nucleus. Similarity: Belongs to the Mediator complex subunit 22 family. SWISS: Q15528 Gene ID: 6837 Database links: Entrez Gene: 417159 Chicken Entrez Gene: 6837 Human Entrez Gene: 20933 Mouse Entrez Gene: 415234 Zebrafish Omim: 185641 Human SwissProt: Q800L3 Chicken SwissProt: Q15528 Human SwissProt: Q62276 Mouse SwissProt: A5WUL3 Zebrafish Unigene: 78354 Human Unigene: 397932 Mouse Unigene: 202721 Rat Unigene: 15892 Zebrafish |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
