| 產品編號 | bs-21046R |
| 英文名稱 | Rabbit Anti-POU2F3 antibody |
| 中文名稱 | 轉錄因子POU2F3抗體 |
| 別 名 | class 2; Epoc 1; Epoc1; HGNC19864; Oct 11; Oct-11; OCT11; Octamer binding transcription factor 11; Octamer-binding protein 11; Octamer-binding transcription factor 11; OTF-11; PLA 1; PLA1; PLA1 protein; PO2F3_HUMAN; POU class 2 homeobox 3; POU domain; POU domain transcription factor OCT11a; POU domain, class 2, transcription factor 3; POU transcription factor; POU2F3; Skn 1a; transcription factor 3; Transcription factor PLA-1; Transcription factor Skn-1; Transcription factor Skn1. |
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Specific References (1) | bs-21046R has been referenced in 1 publications.
[IF=31.745] Zhen Xiong. et al. Intestinal Tuft-2 cells exert antimicrobial immunity via sensing bacterial metabolite N-undecanoylglycine. Immunity. 2022 Mar;: WB ; Mouse.
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| 研究領域 | 腫瘤 細胞生物 轉錄調節因子 細胞分化 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Sheep,Cow,Dog) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 47kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human POU2F3: 1-100/436 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a member of the POU domain family of transcription factors. POU domain transcription factors bind to a specific octamer DNA motif and regulate cell type-specific differentiation pathways. The encoded protein is primarily expressed in the epidermis, and plays a critical role in keratinocyte proliferation and differentiation. The encoded protein is also a candidate tumor suppressor protein, and aberrant promoter methylation of this gene may play a role in cervical cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011] Function: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Regulated the expression of a number of genes such as SPRR2A or placental lactogen. Subcellular Location: Nucleus. Tissue Specificity: Specifically expressed in epidermis and cultured keratinocytes. Similarity: Belongs to the POU transcription factor family. Class-2 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain. SWISS: Q9UKI9 Gene ID: 25833 Database links: Entrez Gene: 25833 Human Entrez Gene: 18988 Mouse Omim: 607394 Human SwissProt: Q9UKI9 Human SwissProt: P31362 Mouse Unigene: 227115 Human Unigene: 325445 Mouse Unigene: 436650 Mouse Unigene: 81060 Rat |
| 產品圖片 |
Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti- POU2F3 (bs-21046R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 48 kD
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti- POU2F3 (bs-21046R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 48 kD
Paraformaldehyde-fixed, paraffin embedded (rat skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (POU2F3) Polyclonal Antibody, Unconjugated (bs-21046R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
