產品編號 | bs-18797R |
英文名稱 | Rabbit Anti-MEST antibody |
中文名稱 | 中胚層特異性轉錄同源蛋白MEST抗體 |
別 名 | DKFZp686L18234; EC 3.-.-.-; Mesoderm specific transcript (mouse) homolog; Mesoderm specific transcript homolog (mouse); Mesoderm-specific transcript homolog protein; MEST; MEST_HUMAN; MGC111102; MGC8703; OTTHUMP00000210362; OTTHUMP00000210364; OTTHUMP00000210367; Paternally expressed gene 1; Paternally-expressed gene 1 protein; PEG1. |
研究領域 | 細胞生物 免疫學 轉錄調節因子 淋巴細胞 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | (predicted: Human,Mouse,Rat,Pig,Cow,Dog,Horse) |
產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 39kDa |
細胞定位 | 細胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human MEST: 101-200/335 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產品介紹 |
This gene encodes a member of the alpha/beta hydrolase superfamily. It is imprinted, exhibiting preferential expression from the paternal allele in fetal tissues, and isoform-specific imprinting in lymphocytes. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promotor switching. The encoded protein may play a role in development. Alternatively spliced transcript variants encoding multiple isoforms have been identified for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3 and 4, and the long arm of chromosomes 6 and 15. [provided by RefSeq, Dec 2011] Subcellular Location: Endoplasmic reticulum membrane. Tissue Specificity: Highly expressed in hydatidiform moles, but barely expressed in dermoid cysts. Biallelic expression is detected in blood lymphocytes. Seems to imprinted in an isoform-specific manner rather than in a tissue-specific manner in lymphocytes. Isoform 1 is expressed only from the paternal allele. Isoform 2 is expressed from both the paternal allele and the maternal allele. Similarity: Belongs to the AB hydrolase superfamily. SWISS: Q5EB52 Gene ID: 4232 Database links: Entrez Gene: 4232 Human Entrez Gene: 17294 Mouse Omim: 601029 Human SwissProt: Q5EB52 Human SwissProt: Q07646 Mouse Unigene: 270978 Human Unigene: 59431 Rat |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |