| 產品編號 | bs-18372R |
| 英文名稱 | Rabbit Anti-LRRC26 antibody |
| 中文名稱 | 富含亮氨酸重復蛋白26抗體 |
| 別 名 | bA350O14.10; CAPC; cytokeratin-associated protein; Cytokeratin-associated protein in cancer; Leucine-rich repeat-containing protein 26; LRC26_HUMAN; Lrrc26. |
| 研究領域 | 腫瘤 細胞生物 免疫學 神經生物學 細胞膜受體 細胞膜蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 32kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LRRC26: 201-300/334 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
LRRC26 is a 334 amino acid protein that localizes to the cytoplasm and contains six LRR repeats. Expressed at high levels in normal colon, pancreas, prostate, salivary gland and intestine, LRRC26 is also present in several cancer cell lines, including colon cancer and pancreatic cancer, suggesting an important role in carcinogenesis. Function: Auxiliary protein of the large-conductance, voltage and calcium-activated potassium channel (BK alpha). Required for the conversion of BK alpha channels from a high-voltage to a low-voltage activated channel type in non-excitable cells. These are characterized by negative membrane voltages and constant low levels of calcium. Subcellular Location: Membrane. Cytoplasm; cytoskeleton. Although predicted to be a single-pass type I membrane protein, this protein localizes to the cytoplasm when expressed at high levels. Tissue Specificity: Isoform 1 is expressed highly in normal prostate and salivary gland, very weakly in colon, pancreas, and intestine, and not at all in other tissues. Isoform 1 is expressed highly in many cancer cell lines and in breast cancer, pancreatic cancer and colon cancer. Isoform 2 is expressed in cancer cell lines. Similarity: Contains 5 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain. SWISS: Q2I0M4 Gene ID: 389816 Database links: Entrez Gene: 389816 Human Omim: 613505 Human SwissProt: Q2I0M4 Human Unigene: 669977 Human |
| 產品圖片 |
Tissue/cell: human laryngo carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-LRRC26 Polyclonal Antibody, Unconjugated(bs-18372R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
