產品編號 | bs-18170R |
英文名稱 | Rabbit Anti-IL34 antibody |
中文名稱 | 白細胞介素34抗體 |
別 名 | C16orf77; Chromosome 16 open reading frame 77; FPT025; IL 34; IL-34; IL34; IL34_HUMAN; Interleukin 34; Interleukin-34; Interleukin34; MGC34647. |
Specific References (2) | bs-18170R has been referenced in 2 publications.
[IF=5.026] Qinghua Liu. et al. Inverse correlation between Interleukin-34 and gastric cancer, a potential biomarker for prognosis. Cell Biosci. 2020 Dec;10(1):1-13 IHC ;
[IF=3.984] Liu, Kehui. et al. Combination of IL-34 and AFP improves the diagnostic value during the development of HBV related hepatocellular carcinoma. Clin Exp Med. 2022 Mar;:1-13 IHC ; Human.
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研究領域 | 細胞生物 免疫學 細胞分化 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Human (predicted: Mouse,Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 25kDa |
細胞定位 | 分泌型蛋白 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human IL34: 21-100/242 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產品介紹 |
Interleukin-34 is a cytokine that promotes the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor (CSF1R; MIM 164770) (Lin et al., 2008 [PubMed 18467591]).[supplied by OMIM, May 2008] Function: Cytokine that promotes the differentiation and viability of monocytes and macrophages. Stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1. Ligand for colony-stimulating factor-1 receptor CSF1R. Subcellular Location: Secreted. Tissue Specificity: Detected in the sinusoidal epithelium in the red pulp of spleen (at protein level). Predominantly expressed in spleen. Also detected in a range of other tissues including heart, brain, lung, liver, kidney, thymus, testis, ovary, small intestine, prostate and colon. Similarity: Belongs to the IL-34 family. SWISS: Q8R1R4 Gene ID: 146433 Database links: Entrez Gene: 146433 Human Entrez Gene: 76527 Mouse Omim: 612081 Human SwissProt: Q6ZMJ4 Human SwissProt: Q8R1R4 Mouse Unigene: 461214 Human Unigene: 77697 Mouse Unigene: 16799 Rat |
產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (human intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL34) Polyclonal Antibody, Unconjugated (bs-18170R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |