| 產品編號 | bs-17302R |
| 英文名稱 | Rabbit Anti-SFRS8 antibody |
| 中文名稱 | 絲氨酸/蘇氨酸SFRS8抗體 |
| 別 名 | 1190005N23Rik; 6330437E22Rik; AI197402; AW212079; MGC167082; SFRS 8; Sfrs8 protein; Splicing factor arginine/serine rich 8 (suppressor of white apricot Drosophila homolog); Splicing factor arginine/serine rich 8 (suppressor of white apricot homolog Drosophila); Splicing factor arginine/serine rich 8; Splicing factor, arginine/serine-rich 8; Suppressor of white apricot protein homolog; SWAP. |
| 研究領域 | 細胞生物 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 105kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SFRS8: 51-150/951 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a human homolog of Drosophila splicing regulatory protein. This gene autoregulates its expression by control of splicing of its first two introns. In addition, it also regulates the splicing of fibronectin and CD45 genes. Two transcript variants encoding different isoforms have been identified. [provided by RefSeq, May 2012] Function: SFRS8 is a human homolog of Drosophila splicing regulatory protein. This gene autoregulates its expression by control of splicing of its first two introns. In addition, it also regulates the splicing of fibronectin and CD45 genes. Multiple alternatively spliced variants have been identified. Two alternatively spliced variants have been characterized to date. Subcellular Location: Nuclear SWISS: Q12872 Gene ID: 6433 Database links: Entrez Gene: 6433 Human Entrez Gene: 231769 Mouse Omim: 601945 Human SwissProt: Q12872 Human SwissProt: Q3USH5 Mouse Unigene: 308171 Human Unigene: 288714 Mouse |
| 產品圖片 |
Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-SFRS8 (bs-17302R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 105 kD
Observed band size: 110 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SFRS8) Polyclonal Antibody, Unconjugated (bs-17302R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SFRS8) Polyclonal Antibody, Unconjugated (bs-17302R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
