| 產品編號 | bs-16872R |
| 英文名稱 | Rabbit Anti-ODCp antibody |
| 中文名稱 | 精氨酸脫羧酶抗體 |
| 別 名 | L-Arginine decarboxylase; ADC; ADC_HUMAN; Antizyme inhibitor 2; ArgDC; Arginine decarboxylase; AZI2; KIAA1945; L-Arginine carboxy lyase; ODC antizyme inhibitor 2; ODC paralog; ODC-p; ODC-paralogue; ODC1L; ODCp; Ornithine decarboxylase like; Ornithine decarboxylase paralog; Ornithine decarboxylase-like protein. |
| 研究領域 | 細胞生物 神經生物學 信號轉導 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 50kDa |
| 細胞定位 | 細胞核 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ODCp: 51-150/460 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
ODCp is a 460 amino acid protein that is expressed in brain and testis. ODCp is a member of the Orn/Lys/Arg decarboxylase class-II family that catalyzes the creation of CO2 and agmatine from L-arginine. Mammalian ODCp differs from the forms expressed in bacteria and plants and shares less than 50% homology with Ornithine decarboxylase (ODC). ODCp is associated with the mitochondrial membrane where excess agmatine can be degraded by the enzyme Agmatinase or bound by the imidazoline recepto. In the brain, the highest levels of ODCp are found in the hypothalamus. Mammalian ODCp is thermally unstable and can be inhibited by Ca2+, Co2+ and polyamines. Function: Decarboxylates L-arginine to agmatine. Truncated splice isoforms probably lack activity. Subunit: Interacts with OAZ1, OAZ2 and OAZ3; the interactions stabilize the complex by inhibiting AZIN2 ubiquitination and degradation. Does not form an heterodimer with ODC1 (By similarity). Monomer. Interaction with OAZ1, OAZ2 and OAZ3; the interactions lead to increased ornithine decarboxylase (ODC)activity and decreased rate of ODC1 degradation. Subcellular Location: Nucleus. Tissue Specificity: Highly expressed in brain. Also expressed in testis. Similarity: Belongs to the Orn/Lys/Arg decarboxylase class-II family. SWISS: Q96A70 Gene ID: 113451 Database links: Entrez Gene: 113451 Human Entrez Gene: 242669 Mouse Omim: 608353 Human SwissProt: Q96A70 Human SwissProt: Q8BVM4 Mouse Unigene: 101807 Human Unigene: 215814 Mouse Unigene: 50921 Rat |
| 產品圖片 |
Sample: Testis (Mouse)Lysate at 40 ug
Primary: Anti-ODCp(bs-16872R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 50kD
Observed band size: 50kD
Sample: Raji (Human)Cell Lysate at 40 ug
Primary: Anti-ODCp(bs-16872R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 50kD
Observed band size: 50kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ODCp) Polyclonal Antibody, Unconjugated (bs-16872R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
