| 產品編號 | bs-16578R |
| 英文名稱 | Rabbit Anti-TRAP5 antibody |
| 中文名稱 | 抗酒石酸酸性磷酸酶5型/5型酸性磷酸酶抗體 |
| 別 名 | Tartrate Resistant Acid Phosphatase; Acid phosphatase 5, tartrate resistant; ACP5; EC 3.1.3.2; PPA5_HUMAN; serum band 5 tartrate-resistant acid phosphatase; SPENCDI; Tartrate-resistant acid ATPase; Tartrate-resistant acid phosphatase type 5; TR-AP; TRACP 5; TRAP; TrATPase; Type 5 acid phosphatase. |
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Specific References (2) | bs-16578R has been referenced in 2 publications.
[IF=7.419] Xiaoyu Cai. et al. Secretory phosphoprotein 1 secreted by fibroblast-like synoviocytes promotes osteoclasts formation via PI3K/AKT signaling in collagen-induced arthritis. BIOMED PHARMACOTHER. 2022 Nov;155:113687 FC ; Mouse.
[IF=6.208] Cong-Jin Liu. et al. Preventing Disused Bone Loss through Inhibition of Advanced Glycation End Products. INT J MOL SCI. 2023 Jan;24(5):4953 IF ; Rat.
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| 研究領域 | 腫瘤 細胞生物 免疫學 信號轉導 淋巴細胞 t-淋巴細胞 b-淋巴細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Pig) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=2ug/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 34kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TRAP5: 171-270/325 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. [provided by RefSeq, Aug 2008] Function: Defects in ACP5 are the cause of spondyloenchondrodysplasia with immune dysregulation (SPENCDI) [MIM:607944]. A disease characterized by vertebral and metaphyseal dysplasia, spasticity with cerebral calcifications, and strong predisposition to autoimmune diseases. The skeletal dysplasia is characterized by radiolucent and irregular spondylar and metaphyseal lesions that represent islands of chondroid tissue within bone. Note=ACP5 inactivating mutations result in a functional excess of phosphorylated osteopontin causing deregulation of osteopontin signaling and consequential autoimmune disease. Subcellular Location: Lysosome. Similarity: Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family. SWISS: P13686 Gene ID: 54 Database links: Entrez Gene: 54 Human Omim: 171640 Human SwissProt: P13686 Human Unigene: 1211 Human |
| 產品圖片 |
Blank control（black line）:Hela.
Primary Antibody (green line): Rabbit Anti-TRAP5 antibody (bs-16578R)
Dilution:2ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
