| 產品編號 | bs-14527R |
| 英文名稱 | Rabbit Anti-EHD3 antibody |
| 中文名稱 | EHD3蛋白抗體 |
| 別 名 | EH domain containing 3; EHD3_HUMAN; EHD3; PAST3. |
| 研究領域 | 細胞生物 轉運蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 61kDa |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EHD3: 11-110/535 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The Eps15 homology (EH) domain-containing protein family consists of four members, EHD1, EHD2, EHD3, and EHD4 (1). The chromosomal locations of the human EHD genes are as follows: EHD1 maps to 11q13, EHD2 maps to 19q13.3, EHD3 maps to 2p21, and EHD4 maps to 15q11.1 (1-3). The encoded proteins of all EHD family members contain multiple conserved regions, which include an amino-terminal nucleotide-binding consensus site, a bipartite nuclear localization signal, and a carboxy-terminal EH protein-binding domain with an EF-hand motif (3,4). EHD1 is ubiquitously expressed with increased expression in testis (2,3). EHD2, EHD3, and EHD4 have more specific expression with EHD2 highly expressed in heart, EHD3 expressed in brain, kidney, liver, placenta, ovary, and heart, and EHD4 expressed in heart, placenta, and pancreas (1,5,6). The EHD proteins may participate in ligand-induced endocytosis (3-5). Function: EHD3 contains one EF-hand domain and one EH domain. It plays a role in endocytic transport. EHD3 is highly expressed in heart and brain and moderately expressed in kidney, liver and placenta. Subunit: Homooligomer, and heterooligomer with EHD1, EHD2 and EHD4. Interacts with PACSIN1 and PACSIN2. Interacts (via EH domain) with MICALL1. Subcellular Location: Endosome membrane, cytoplasmic side. Nucleus. Tissue Specificity: Highly expressed in heart and brain and moderately expressed in kidney, liver, and placenta. Similarity: Contains 1 EF-hand domain. Contains 1 EH domain. SWISS: Q9NZN3 Gene ID: 30845 Database links: Entrez Gene: 30845 Human Entrez Gene: 57440 Mouse Omim: 605891 Human SwissProt: Q9NZN3 Human SwissProt: Q9QXY6 Mouse |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EHD3) Polyclonal Antibody, Unconjugated (bs-14527R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
