| 產品編號 | bs-4314R |
| 英文名稱 | Rabbit Anti-EIF1AY antibody |
| 中文名稱 | 真核翻譯起始因子1A抗體 |
| 別 名 | eIF 1A Y isoform; eIF 4C; eIF-1A Y isoform; EIF-4C; EIF1AY; Eukaryotic translation initiation factor 1A; Eukaryotic translation initiation factor 1A, Y chromosomal; Eukaryotic translation initiation factor 1A, Y linked; Eukaryotic translation initiation factor 4C; IF1AY_HUMAN; Y-chromosomal. |
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Specific References (1) | bs-4314R has been referenced in 1 publications.
[IF=4.243] Rongxin Geng. et al. EIF1A depletion restrains human pituitary adenoma progression. Transl Oncol. 2022 Jan;15:101299 WB,IHC ; Human,Mouse.
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| 研究領域 | 細胞生物 轉錄調節因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Cow) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 16kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EIF1AY: 21-120/144 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene is located on the non-recombining region of the Y chromosome. It encodes a protein related to eukaryotic translation initiation factor 1A (EIF1A), which may function in stabilizing the binding of the initiator Met-tRNA to 40S ribosomal subunits. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] Function: Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits. Tissue Specificity: Ubiquitous. Similarity: Belongs to the eIF-1A family. Contains 1 S1-like domain. SWISS: O14602 Gene ID: 9086 Database links: Entrez Gene: 9086 Human Omim: 400014 Human SwissProt: O14602 Human Unigene: 461178 Human |
| 產品圖片 |
Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-EIF1AY (bs-4314R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 16 kD
Observed band size: 18 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EIF1AY) Polyclonal Antibody, Unconjugated (bs-4314R ) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EIF1AY) Polyclonal Antibody, Unconjugated (bs-4314R ) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
