| 產(chǎn)品編號(hào) | bs-13603R |
| 英文名稱 | Rabbit Anti-ZMYM2 antibody |
| 中文名稱 | 鋅指蛋白198抗體 |
| 別 名 | FIM; Fused in myeloproliferative disorders protein; MYM; RAMP; Rearranged in atypical myeloproliferative disorder protein; SCLL; ZFP 198; Zinc finger MYM type protein 2; Zinc finger MYM-type protein 2; Zinc finger protein 198; Zinc finger protein198; zinc finger, MYM-type 2; ZMYM 2; ZMYM2; ZMYM2_HUMAN; ZNF 198; ZNF198. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 干細(xì)胞 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse,Rabbit) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 160kDa |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ZMYM2/ZNF198: 1051-1150/1377 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZNF198 (zinc finger 198), also known as ZMYM2 (zinc finger, MYM-type 2), FIM, MYM, RAMP or SCLL, is a 1,377 amino acid protein that localizes to the nucleus and contains nine MYM-type zinc fingers. Thought to be a component of the BHC histone deacetylase complex, ZNF198 interacts with HDAC1 and HDAC2 and is thought to stabilize the BHC complex via its MYM-type zinc fingers. The gene encoding ZNF198 is subject to a translocation with Flg, an event that may be involved in the pathogenesis of stem cell leukemia lymphoma syndrome (SCLL), a lymphoblastic lymphoma often accompanied by pronounced peripheral eosinophilia and/or prominent eosinophilic infiltrates in the affected bone marrow. Function: May function as a transcription factor. Subcellular Location: Nucleus. DISEASE: Note=A chromosomal aberration involving ZMYM2 may be a cause of stem cell leukemia lymphoma syndrome (SCLL). Translocation t(8;13)(p11;q12) with FGFR1. SCLL usually presents as lymphoblastic lymphoma in association with a myeloproliferative disorder, often accompanied by pronounced peripheral eosinophilia and/or prominent eosinophilic infiltrates in the affected bone marrow. Similarity: Contains 9 MYM-type zinc fingers. SWISS: Q9CU65 Gene ID: 7750 Database links: Entrez Gene: 7750 Human Entrez Gene: 76007 Mouse Omim: 602221 Human SwissProt: Q9UBW7 Human SwissProt: Q9CU65 Mouse Unigene: 507433 Human Unigene: 31417 Mouse |
| 產(chǎn)品圖片 |
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ZMYM2 Polyclonal Antibody, Unconjugated(bs-13603R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ZMYM2 Polyclonal Antibody, Unconjugated(bs-13603R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZMYM2) Polyclonal Antibody, Unconjugated (bs-13603R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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