| 產品編號 | bs-15411R |
| 英文名稱 | Rabbit Anti-HAPLN3 antibody |
| 中文名稱 | 透明質酸及粘蛋白3抗體 |
| 別 名 | EXLD1; Extracellular link domain containing, 1; HAPLN3; HPLN3_HUMAN; HsT19883; Hyaluronan and proteoglycan link protein 3. |
| 研究領域 | 信號轉導 細菌及病毒 細胞外基質 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Pig,Horse,Chimpanzee) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=2ug/Test,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 39kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HAPLN3: 51-150/360 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
HAPLN3 is a 360 amino acid protein encoded by the human gene HAPLN3. HAPLN3 belongs to the HAPLN family and contains 1 Ig-like V-type (immunoglobulin-like) domain and two Link domains. HAPLN3 mediates the binding of complexes containing hyaluronic acid. May play a pivotal role in the formation of the hyaluronan-associated matrix in the central nervous system (CNS) which facilitates neuronal conduction and general structural stabilization. HAPLN3 may also be involved in the formation of extracellular matrices contributing to perineuronal nets and facilitate the understanding of a functional role of these extracellular matrices. HAPLN3 is widely expressed with highest levels in spleen and placenta. Function: May function in hyaluronic acid binding. Subcellular Location: Secreted, extracellular space, extracellular matrix (By similarity). Tissue Specificity: Widely expressed with highest levels in spleen and placenta. Similarity: Belongs to the HAPLN family. Contains 1 Ig-like V-type (immunoglobulin-like) domain. Contains 2 Link domains SWISS: Q96S86 Gene ID: 145864 Database links: Entrez Gene: 145864 Human SwissProt: Q96S86 Human Unigene: 447530 Human |
| 產品圖片 |
Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-HAPLN3 antibody (bs-15411R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ethanol (10min at room temperature) and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
