| 產品編號 | bs-13513R |
| 英文名稱 | Rabbit Anti-GPR110 antibody |
| 中文名稱 | G蛋白偶聯受體110抗體 |
| 別 名 | G protein coupled receptor 110; G protein coupled receptor KPG_012; G protein coupled receptor PGR19; G-protein coupled receptor KPG_012; G-protein coupled receptor PGR19; GP110_HUMAN; GPR110; HGPCR36; KPG_012; PGR19; Probable G protein coupled receptor 110; Probable G-protein coupled receptor 110; GPCR GPR110. |
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Specific References (1) | bs-13513R has been referenced in 1 publications.
[IF=4.125] Minghui Zhang. et al. Comparative Transcriptomic Analysis of Mammary Gland Tissues Reveals the Critical Role of GPR110 in Palmitic Acid-Stimulated Milk Protein and Fat Synthesis. BRIT J NUTR. 2023 Mar;:1-32 WB ; Mouse.
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| 研究領域 | 細胞生物 神經生物學 信號轉導 G蛋白偶聯受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg/Test,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 99kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human G protein coupled receptor 110: 51-150/910 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
G protein-coupled receptors (GPRs), also known as seven transmembrane receptors, heptahelical receptors or 7TM receptors, comprise a superfamily of proteins that play a role in many different stimulus-response pathways. G protein coupled receptors translate extracellular signals into intracellular signals (G protein activation) and they respond to a variety of signaling molecules, such as hormones and neurotransmitters. GPR110 (G protein-coupled receptor 110), also known as PGR19, is a 911 amino acid protein that belongs to the G-protein coupled receptor 2 family and LN-TM7 subfamily. Characterized as an adhesion GPCR, GPR110 is a multipass membrane-bound protein with a long amino-terminus that contains multiple domains. One of these domains is the GPCR proteolytic site (GPS), which is essential for proteolytic cleavage of the amino-terminus and for cell surface expression. Function: Orphan receptor. Subcellular Location: Cell membrane. Similarity: Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. Contains 1 GPS domain. Contains 1 SEA domain. SWISS: Q5T601 Gene ID: 266977 Database links: Entrez Gene: 266977 Human SwissProt: Q5T601 Human Unigene: 733762 Human |
| 產品圖片 |
Sample: A549 (human)Cell Lysate at 40 ug
Primary: Anti-GPR110(bs-13513R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 99 kD
Observed band size: 109 kD
Sample: A431 (human)Cell Lysate at 40 ug
Primary: Anti-GPR110(bs-13513R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 99 kD
Observed band size: 109 kD
Blank control(blue): Mouse Kidney Cells(fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti- GPR110 antibody(bs-13513R), Dilution: 1ug in 100 uL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
