| 產品編號 | bs-13378R |
| 英文名稱 | Rabbit Anti-COLGALT1 antibody |
| 中文名稱 | 糖基轉移酶25結構域1/前膠原半乳糖基轉移酶1抗體 |
| 別 名 | GLT25D1; FLJ22329; Glycosyltransferase 25 domain containing 1; Hydroxylysine galactosyltransferase 1; Procollagen galactosyltransferase 1; GT251_HUMAN. |
| 研究領域 | 細胞生物 信號轉導 新陳代謝 細胞外基質 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Pig,Sheep,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,Flow-Cyt=2ug/Test,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 69kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GLT25D1: 531-622/622 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
GLT25D1 is a 622 amino acid protein that localizes to the lumen of the endoplasmic reticulum. Ubiquitously expressed with higher levels in placenta, heart, lung and spleen, GLT25D1 is a member of the glycosyltransferase 25 family. The beta-galactosyltransferase activity of GLT25D1 allows the transfer of beta-galactose to hydroxylysine residues of collagen. GLT25D1 is considered an important target for investigating the biological significance of collagen glycosylation and the importance of posttranslational modification in the etiology of connective tissue disorders. The gene encoding GLT25D1 is located on human chromosome 19, which consists of over 63 million bases, houses approximately 1,400 genes and is recognized for having the greatest gene density of the human chromosomes. Function: Has a beta-galactosyltransferase activity; transfers beta-galactose to hydroxylysine residues of collagen. Subcellular Location: Endoplasmic reticulum lumen. Note: Colocalized with PLOD3 and mannose binding lectin. Tissue Specificity: Ubiquitous with higher levels in placenta, heart, lung and spleen. Post-translational modifications: N-glycosylated. Similarity: Belongs to the glycosyltransferase 25 family. SWISS: Q8NBJ5 Gene ID: 79709 Database links: Entrez Gene: 100070236 Horse Entrez Gene: 79709 Human Entrez Gene: 234407 Mouse SwissProt: Q8NBJ5 Human |
| 產品圖片 |
Sample:
Lane 1: Mouse Placenta tissue lysates
Lane 2: Mouse Liver tissue lysates
Lane 3: Rat Placenta tissue lysates
Lane 4: Rat Spleen tissue lysates
Lane 5: Rat Liver tissue lysates
Lane 6: Human THP-1 cell lysates
Primary: Anti-COLGALT1 (bs-13378R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69 kDa
Observed band size: 68 kDa
Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-GLT25D1 antibody (bs-13378R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ethanol (10min at room temperature) and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
