產品編號 | bs-13252R |
英文名稱 | Rabbit Anti-G3BP2 antibody |
中文名稱 | G3BP2蛋白抗體 |
別 名 | G3BP 2; G3BP-2; G3BP2; G3BP2_HUMAN; GAP SH3 domain binding protein 2; GAP SH3 domain-binding protein 2; GTPase activating protein (SH3 domain) binding protein 2; Ras GTPase activating protein SH3 domain binding protein 2; Ras GTPase-activating protein-binding protein 2. |
研究領域 | 細胞生物 表觀遺傳學 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse) |
產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 54kDa |
細胞定位 | 細胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human G3BP2: 21-120/482 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產品介紹 |
G3BP2 (GTPase activating protein (SH3 domain) binding protein 2) is a 482 amino acid protein that localizes to the cytoplasm and contains one NTF2 domain and one RRM domain. Existing as two alternatively spliced isoforms, G3BP2 acts as a scaffold protein that is thought to be involved in mRNA transport and is subject to post-translational methylation on select arginine residues. The gene encoding G3BP2 maps to human chromosome 4, which encodes nearly 6% of the human genome and has the largest gene deserts (regions of the genome with no protein encoding genes) of all of the human chromosomes. Defects in some of the genes located on chromosome 4 are associated with Huntington's disease, Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease. Function: Probable scaffold protein that may be involved in mRNA transport. Subunit: Binds to the N-terminal domain of I-kappa-B-alpha. Subcellular Location: Cytoplasm. Post-translational modifications: Arg-457 and Arg-468 are dimethylated, probably to asymmetric dimethylarginine. Similarity: Contains 1 NTF2 domain. Contains 1 RRM (RNA recognition motif) domain. SWISS: Q9UN86 Gene ID: 9908 Database links: Entrez Gene: 9908 Human Entrez Gene: 23881 Mouse SwissProt: Q9UN86 Human SwissProt: P97379 Mouse SwissProt: Q5R9L3 Orangutan Unigene: 303676 Human Unigene: 290530 Mouse Unigene: 473827 Mouse |
產品圖片 |
Sample:
Brain (Mouse) Lysate at 40 ug
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-G3BP2 (bs-13252R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD
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1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |