| 產品編號 | bs-13228R |
| 英文名稱 | Rabbit Anti-Furin antibody |
| 中文名稱 | 弗林蛋白酶抗體 |
| 別 名 | FURIN_HUMAN; Furin; EC:3.4.21.75; FURIN; FUR; PACE; PCSK3; Dibasic-processing enzyme; Paired basic amino acid residue-cleaving enzyme (PACE); |
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Specific References (3) | bs-13228R has been referenced in 3 publications.
[IF=4.238] Takashi Mori. et al. Gallic Acid is a Dual α/β-Secretase Modulator that Reverses Cognitive Impairment and Remediates Pathology in Alzheimer Mice. J Biol Chem. 2020 Nov;295:16251 WB ; Mouse.
[IF=3.355] Samuel James Brake. et al. SARS-CoV-2 (COVID-19) Adhesion Site Protein Upregulation in Small Airways, Type 2 Pneumocytes, and Alveolar Macrophages of Smokers and COPD – Possible Implications for Interstitial Fibrosis. Int J Chronic Obstr. 2022 Jan;17:101-115 IF,IHC ; Human.
[IF=1.376] Gentaro Yamasaki. et al. Effect of FURIN SNP rs17514846 on coronary atherosclerosis in human cardiac specimens: An autopsy study of 106 cases. Legal Med-Tokyo. 2022 Mar;55:102006 IHC ; Human.
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| 研究領域 | 細胞生物 信號轉導 新陳代謝 泛素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 74kDa |
| 細胞定位 | 細胞漿 細胞膜 細胞外基質 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Furin: 401-500/794 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Furin is a calcium-dependent serine endoprotease that belongs to the subtilisin-like proprotein convertase family. The members of this family process latent precursor proteins into their biologically active products. Furin cleaves at paired basic amino acid processing sites within proparathyroid hormone, transforming growth factor β 1 precursor, proalbumin, pro-β-secretase, membrane type-1 matrix metalloproteinase, β subunit of pro-nerve growth factor and von Willebrand factor. Furin can directly cleave proMMP-2 within the ttrans-Golgi network leading to an inactive form of matrix metalloproteinase-2 (MMP-2). Furin is synthesized as an inactive zymogen that may minimize the occurrence of premature enzymatic activity that would lead to alternative protein activation or degradation. The inhibitory mechanism is based on the presence of an inactivating prosegment at the NH2 terminal of the Furin. After initial autocatalytic cleavage, the prosegment remains tightly associated until it reaches the trans-Golgi network where the dissociation of the prosegment and activation of furin occurs. Function: Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. Subunit: Interacts with FLNA (By similarity). Binds to PACS1 which mediates TGN localization and connection to clathrin adapters. Subcellular Location: Golgi apparatus > trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin. Tissue Specificity: Seems to be expressed ubiquitously. Post-translational modifications: The inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation. Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms. Similarity: Belongs to the peptidase S8 family. Furin subfamily. Contains 1 homo B/P domain. SWISS: P09958 Gene ID: 5045 Database links: Entrez Gene: 5045 Human Entrez Gene: 18550 Mouse SwissProt: P09958 Human SwissProt: P23188 Mouse 在真核生物細胞中,許多具有生物活性的多肽和蛋白是在其分泌過程中由前體蛋白經內切蛋白酶切割后激活形成的.弗林蛋白酶(Furin)就是這個內切蛋白酶家族重要成員之一,它可以識別剪切多種蛋白質,如生長醫子、血清蛋白、基質金屬蛋白酶、受體、病毒囊膜蛋白和細菌外毒素等.近年來Furin得到了迅速而廣泛的研究,本文簡介了它的表達與加工運輸、生物學功能、與病毒侵染的關系,以及它的抑制劑. |
| 產品圖片 |
Tissue/cell: mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Furin Polyclonal Antibody, Unconjugated(bs-13228R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
